Fukano Hanako, Yoshida Mitsunori, Kazumi Yuko, Fujiwara Nagatoshi, Katayama Kinya, Ogura Yoshitoshi, Hayashi Tetsuya, Miyamoto Yuji, Fujimoto Noriki, Hongsheng Wang, Mizumoto Chisaki, Koizumi Yusuke, Maeda Hiroyoshi, Hiranuma Osamu, Mitarai Satoshi, Ishii Norihisa, Hoshino Yoshihiko
1Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba, Higashi-Murayama, Tokyo, Japan.
2Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo, Japan.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2437-2442. doi: 10.1099/ijsem.0.002845. Epub 2018 Jun 25.
Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of the largest groups, consisting of 18 species of slow-growing mycobacteria. In 2009, a case of NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on the chest, back and extremities, and an M. simiae-like organism was isolated from skin biopsy specimens obtained from one of these lesions. Based on several assessments, including multiple-gene analyses, biochemical characterization and drug susceptibility testing, we concluded that this isolate represented a novel species of NTM, and proposed the name 'Mycobacterium shigaense'. Since 2009, five more cases of NTM-associated infectious disease in which there was a suspected involvement of 'M. shigaense' have been reported. Interestingly, four of these six cases occurred in Shiga Prefecture. Here we performed multiple-gene phylogenetic analyses, physiological and biochemical characterization tests, drug susceptibility tests, and profiling of proteins, fatty acids and mycolic acids of eight clinical isolates from the six suspected 'M. shigaense' cases. The results confirmed that all of the clinical isolates were 'M. shigaense', a slow-growing, scotochromogenic species. Here M. shigaense is validly proposed as a new member of the M. simiae complex, with the type strain being UN-152 (=JCM 32072=DSM 46748).
在非结核分枝杆菌（NTM）中，猿分枝杆菌复合体是最大的类群之一，由18种生长缓慢的分枝杆菌组成。2009年，日本滋贺县报告了1例NTM相关的感染性皮肤病病例。患者胸部、背部和四肢出现散在结节，从其中一个皮损的皮肤活检标本中分离出一种类似猿分枝杆菌的菌株。基于包括多基因分析、生化特性鉴定和药敏试验在内的多项评估，我们得出结论，该分离株代表一种新的NTM菌种，并提议将其命名为“滋贺分枝杆菌”。自2009年以来，又报告了5例疑似“滋贺分枝杆菌”感染的NTM相关传染病病例。有趣的是，这6例病例中有4例发生在滋贺县。在此，我们对来自6例疑似“滋贺分枝杆菌”病例的8株临床分离株进行了多基因系统发育分析、生理生化特性鉴定试验、药敏试验以及蛋白质、脂肪酸和分枝菌酸分析。结果证实，所有临床分离株均为“滋贺分枝杆菌”，这是一种生长缓慢的暗产色菌种。在此，滋贺分枝杆菌被正式提议作为猿分枝杆菌复合体的一个新成员，模式菌株为UN-152（=JCM 32072=DSM 46748）。
