Es-sette Nargys, Ajaoud Malika, Anga Latifa, Mellouki Fouad, Lemrani Meryem
Laboratoire de Parasitologie et Maladies Vectorielles, Institut Pasteur du Maroc, Casablanca, Morocco.
Laboratoire de Chimie Physique et Bioorganique, URAC C22, Faculté des Sciences et Techniques, Université Hassan II Casablanca, Mohammedia, Morocco.
Parasit Vectors. 2015 Apr 3;8:205. doi: 10.1186/s13071-015-0826-1.
To investigate the transmission of phleboviruses, a total of 7,057 sandflies were collected in well-known foci of cutaneous leishmaniasis and were identified to species level according to morphological characters.Collected sandflies were tested by Nested PCR for the presence of Phleboviruses and subsequently by viral isolation on Vero cells. The corresponding products were sequenced. Toscana virus was isolated, for the first time, from 5 pools of sandflies.Hence, Toscana virus should be considered a potential risk that threatens public health and clinicians should be aware of the role of Toscana virus in cases of meningitis and encephalitis in Morocco.
为了研究白蛉病毒的传播情况,在已知的皮肤利什曼病疫源地共采集了7057只白蛉,并根据形态特征将其鉴定到种水平。对采集的白蛉进行巢式PCR检测以确定是否存在白蛉病毒,随后在Vero细胞上进行病毒分离。对相应产物进行测序。首次从5组白蛉中分离出托斯卡纳病毒。因此,托斯卡纳病毒应被视为威胁公众健康的潜在风险,临床医生应意识到托斯卡纳病毒在摩洛哥脑膜炎和脑炎病例中的作用。