Zarrin Majid, Rahdar Mahmoud, Gholamian Abbas
Health research Institute, Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Cellular and Molecular Researches Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Jundishapur J Microbiol. 2015 Mar 21;8(3):e17614. doi: 10.5812/jjm.17614. eCollection 2015 Mar.
Biological control of parasitic nematodes by microorganisms is a promising approach to control such parasites. Microorganisms such as fungi, viruses and bacteria are recognized as biocontrol agents of nematodes.
The current study mainly aimed to evaluate the in vitro Potential of various saprophyte soil-fungi in reducing the infective larvae stage of parasitic nematode Trichostrongylidae family.
Sheep feces were employed to provide the required third stage larvae source for the experiments. The nematode infective larvae of Trichostrongylidae family including three species of Ostertagia circumcincta, Marshalgia marshali and Heamonchos contortus were collected by Berman apparatus. Fifteen isolates of filamentous fungi were tested in the current study. One milliliter suspension containing 200 third stage larvae of Trichostrongylidae family was separately added to the fungal cultures in 2% water-agar medium Petri-dishes. Every day the live larvae were counted with light microscope (10X) and the number of captured larvae was recorded on different days.
Significant differences were observed in the results of co-culture of nematodes larva and fungi after seven days. The most effective fungi against the nematodes larvae were Cladosporium sp., Trichoderma sp., Fusarium equisetti, after seven days of incubation.
The studies on fungi could be applied as suitable tools in biocontrol of nematode infections. However, additional surveys are required to select efficient with the ability to reduce the nematode larvae in the environment.
利用微生物对寄生线虫进行生物防治是控制此类寄生虫的一种有前景的方法。真菌、病毒和细菌等微生物被认为是线虫的生物防治剂。
本研究主要旨在评估各种腐生土壤真菌在体外降低寄生线虫毛圆科感染性幼虫阶段的潜力。
使用绵羊粪便为实验提供所需的第三阶段幼虫来源。通过伯曼装置收集毛圆科线虫感染性幼虫，包括三种环形奥斯特他线虫、马氏马歇尔线虫和捻转血矛线虫。本研究测试了15种丝状真菌分离株。将含有200条毛圆科第三阶段幼虫的1毫升悬浮液分别添加到2%水琼脂培养基培养皿中的真菌培养物中。每天用光学显微镜（10倍）对活幼虫进行计数，并记录不同天数捕获的幼虫数量。
线虫幼虫与真菌共培养七天后，结果存在显著差异。孵育七天后，对幼虫最有效的真菌是枝孢属、木霉属、平脐蠕孢菌。
关于真菌的研究可作为线虫感染生物防治中的合适工具。然而，需要进一步的调查来选择能够在环境中减少线虫幼虫的有效真菌。
