Evangelista Maristella S, Perez Mireya, Salibian Ara A, Hassan Jeffrey M, Darcy Sean, Paydar Keyianoosh Z, Wicker Ryan B, Arcaute Karina, Mann Brenda K, Evans Gregory R D
Department of Plastic Surgery, University of California, Irvine, Orange, California.
W.M. Keck Center for 3D Innovation, University of Texas at El Paso, El Paso, Texas.
J Reconstr Microsurg. 2015 Jun;31(5):327-35. doi: 10.1055/s-0034-1395415. Epub 2015 Apr 20.
The use of nerve conduits to facilitate nerve regrowth after peripheral nerve injury is limited to defects less than 3 cm. The purpose of this study is to determine the capability of novel single and multi-lumen poly(ethylene glycol) (PEG) conduits manufactured by stereolithography to promote peripheral nerve regeneration.
Eight Sprague Dawley rats with sharp transection injuries of the sciatic nerve were randomly assigned to receive single-lumen or multi-lumen PEG conduits to bridge a 10-mm gap. Sciatic nerve and conduit samples were harvested after 5 weeks, and axon number, myelin thickness, fiber diameter, and g-ratio were analyzed. The contralateral intact nerve was also harvested for comparison.
Partial nerve regeneration was observed in three out of four single-lumen conduits and one out of four multi-lumen conduits. Axon number in the single-lumen regenerated nerve approached that of the contralateral intact nerve at 4,492 ± 2,810.0 and 6,080 ± 627.9 fibers/mm(2), respectively. The percentage of small fibers was greater in the single-lumen conduit compared with the intact nerve, whereas myelin thickness and g-ratio were consistently greater in the autologous nerve. Axon regrowth through the multi-lumen conduits was severely limited.
Single-lumen stereolithography-manufactured PEG nerve conduits promote nerve regeneration, with regenerating axon numbers approaching that of normal nerve. Multi-lumen conduits demonstrated significantly less nerve regeneration, possibly due to physical properties of the conduit inhibiting growth. Further studies are necessary to compare the efficacy of the two conduits for functional recovery and to elucidate the reasons underlying their differences in nerve regeneration potential.
使用神经导管促进周围神经损伤后神经再生，目前仅限于缺损小于3厘米的情况。本研究的目的是确定通过立体光刻制造的新型单腔和多腔聚乙二醇（PEG）导管促进周围神经再生的能力。
八只坐骨神经锐器横断伤的Sprague Dawley大鼠被随机分配接受单腔或多腔PEG导管来桥接10毫米的间隙。5周后采集坐骨神经和导管样本，分析轴突数量、髓鞘厚度、纤维直径和g比值。同时采集对侧完整神经用于比较。
在四个单腔导管中有三个观察到部分神经再生，四个多腔导管中有一个观察到部分神经再生。单腔再生神经中的轴突数量分别为4,492 ± 2,810.0和6,080 ± 627.9根纤维/平方毫米，接近对侧完整神经。与完整神经相比，单腔导管中小纤维的百分比更高，而自体神经中的髓鞘厚度和g比值始终更高。通过多腔导管的轴突再生受到严重限制。
通过立体光刻制造的单腔PEG神经导管可促进神经再生，再生轴突数量接近正常神经。多腔导管的神经再生明显较少，可能是由于导管的物理特性抑制了生长。有必要进一步研究比较两种导管在功能恢复方面的疗效，并阐明它们在神经再生潜力方面存在差异的原因。
