Wang Jǖgang, Zheng Rong, Bai Shulan, Gao Xiaomin, Liu Min, Yan Wei
College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China; College of Life Science and Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia, China.
PLoS One. 2015 Apr 20;10(4):e0124442. doi: 10.1371/journal.pone.0124442. eCollection 2015.
Prunus mongolica Maxim, which is widely established in the Gobi Desert, shows extreme tolerance to drought. However, there is a lack of available transcriptomic resources for this species related to its response to water deficiency. To investigate the mechanisms that allow P. mongolica to maintain growth in extremely arid environments, the response of P. mongolica seedlings to drought stress was analyzed using morphological, physiological, biochemical and high-throughput sequencing approaches. We generated 28,713,735 and 26,650,133 raw reads from no-stress control and drought-stressed P. mongolica seedlings, respectively. In total, we obtained 67,352 transcripts with an average length of 874.44 bp. Compared with the no-stress control, 3,365 transcripts were differentially expressed in the drought-stressed seedlings, including 55.75% (1,876 transcripts) up-regulated and 44.25% (1,489 transcripts) down-regulated transcripts. The photosynthesis response showed a decreasing tendency under drought stress, but the changes in the levels of hormones (auxins, cytokinins and abscisic acid) resulted in the closing of stomata and decreased cell enlargement and division; these changes were effective for promoting P. mongolica survival in Gobi Desert. Next, we analyzed the aquaporin and superoxide dismutase gene families due to their importance in plant resistance to drought stress. We found that all of the plasma membrane intrinsic protein transcripts were down-regulated in the drought-stressed treatment, whereas drought did not affect the expression of nodulin intrinsic protein or small basic intrinsic protein transcripts in P. mongolica seedlings. In addition, activation of iron superoxide dismutase transcription and enhanced transcription of manganese superoxide dismutase were observed in P. mongolica to promote tolerance of drought stress. This study identified drought response genes in P. mongolica seedlings. Our results provide a significant contribution to the understanding of how P. mongolica responds to drought stress at the transcriptome level, which may help to elucidate molecular mechanisms associated with the drought response of almond plants.
蒙古扁桃广泛分布于戈壁沙漠，对干旱具有极强的耐受性。然而，目前缺乏该物种应对水分亏缺的转录组资源。为了探究蒙古扁桃在极端干旱环境中维持生长的机制，采用形态学、生理学、生物化学和高通量测序方法分析了蒙古扁桃幼苗对干旱胁迫的响应。我们分别从无胁迫对照和干旱胁迫的蒙古扁桃幼苗中生成了28,713,735和26,650,133条原始读数。总共获得了67,352条转录本，平均长度为874.44 bp。与无胁迫对照相比，干旱胁迫幼苗中有3,365条转录本差异表达，其中上调转录本占55.75%（1,876条），下调转录本占44.25%（1,489条）。干旱胁迫下光合作用响应呈下降趋势，但激素（生长素、细胞分裂素和脱落酸）水平的变化导致气孔关闭，细胞增大和分裂减少；这些变化有效地促进了蒙古扁桃在戈壁沙漠中的存活。接下来，由于水通道蛋白和超氧化物歧化酶基因家族在植物抗旱胁迫中的重要性，我们对其进行了分析。我们发现，干旱胁迫处理下所有质膜内在蛋白转录本均下调，而干旱对蒙古扁桃幼苗中结节内在蛋白或小碱性内在蛋白转录本的表达没有影响。此外，在蒙古扁桃中观察到铁超氧化物歧化酶转录的激活和锰超氧化物歧化酶转录的增强，以促进对干旱胁迫的耐受性。本研究鉴定了蒙古扁桃幼苗中的干旱响应基因。我们的结果为理解蒙古扁桃在转录组水平上如何响应干旱胁迫做出了重要贡献，这可能有助于阐明与扁桃属植物干旱响应相关的分子机制。
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