College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
Plant Cell Physiol. 2021 Feb 4;61(12):2153-2166. doi: 10.1093/pcp/pcaa137.
Rose (Rosa chinensis) is the most important ornamental crops worldwide. However, the physiological and molecular mechanism of rose under drought stress remains elusive. In this study, we analyzed the changes of photosynthetic and phytohormone levels in the leaves and roots of rose seedlings grown under control (no drought), mild drought (MD) and severe drought stress. The total chlorophyll content and water use efficiency were significantly enhanced under MD in rose leaves. In addition, the concentration of ABA was higher in the leaves compared to the roots, whereas the roots accumulated more IAA, methylindole-3-acetic acid and indole-3-propionic acid. We also constructed the first full-length transcriptome for rose, and identified 96,201,862 full-length reads of average length 1,149 bp that included 65,789 novel transcripts. A total of 3,657 and 4,341 differentially expressed genes (DEGs) were identified in rose leaves and roots respectively. KEGG pathway analysis showed enrichment of plant hormone, signal transduction and photosynthesis are among the DEGs. 42,544 alternatively spliced isoforms were also identified, and alternative 3' splice site was the major alternative splicing (AS) event among the DEGs. Variations in the AS patterns of three genes between leaves and roots indicated the possibility of tissue-specific posttranscriptional regulation in response to drought stress. Furthermore, 2,410 novel long non-coding RNAs were detected that may participate in regulating the drought-induced DEGs. Our findings identified previously unknown splice sites and new genes in the rose transcriptome, and elucidated the drought stress-responsive genes as well as their intricate regulatory networks.
玫瑰（Rosa chinensis）是世界上最重要的观赏作物之一。然而，玫瑰在干旱胁迫下的生理和分子机制仍不清楚。在本研究中，我们分析了在对照（无干旱）、轻度干旱（MD）和严重干旱胁迫下生长的玫瑰幼苗叶片和根系中光合和植物激素水平的变化。在 MD 下，玫瑰叶片中的总叶绿素含量和水分利用效率显著提高。此外，叶片中的 ABA 浓度高于根部，而根部积累了更多的 IAA、甲基吲哚-3-乙酸和吲哚-3-丙酸。我们还构建了第一个玫瑰全长转录组，鉴定出平均长度为 1,149 bp 的 96,201,862 条全长读长，其中包括 65,789 条新转录本。在玫瑰叶片和根部分别鉴定到 3,657 和 4,341 个差异表达基因（DEGs）。KEGG 通路分析显示，植物激素、信号转导和光合作用等途径在 DEGs 中富集。还鉴定到 42,544 个可变剪接异构体，其中 3' 剪接位点的可变剪接（AS）事件是 DEGs 中的主要事件。三个基因在叶片和根之间的 AS 模式的变化表明，在应对干旱胁迫时，可能存在组织特异性的转录后调控。此外，检测到 2,410 个新的长非编码 RNA，它们可能参与调控干旱诱导的 DEGs。我们的研究结果确定了玫瑰转录组中以前未知的剪接位点和新基因，并阐明了干旱胁迫响应基因及其复杂的调控网络。
