Walker Peter J, Widen Steven G, Firth Cadhla, Blasdell Kim R, Wood Thomas G, Travassos da Rosa Amelia P A, Guzman Hilda, Tesh Robert B, Vasilakis Nikos
CSIRO Biosecurity, Australian Animal Health Laboratory, Geelong, Victoria, Australia; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas; Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, The University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, Texas
CSIRO Biosecurity, Australian Animal Health Laboratory, Geelong, Victoria, Australia; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas; Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, The University of Texas Medical Branch, Galveston, Texas; Center for Tropical Diseases, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, Texas.
Am J Trop Med Hyg. 2015 Nov;93(5):1041-51. doi: 10.4269/ajtmh.15-0344. Epub 2015 Aug 31.
The genus Nairovirus of arthropod-borne bunyaviruses includes the important emerging human pathogen, Crimean-Congo hemorrhagic fever virus (CCHFV), as well as Nairobi sheep disease virus and many other poorly described viruses isolated from mammals, birds, and ticks. Here, we report genome sequence analysis of six nairoviruses: Thiafora virus (TFAV) that was isolated from a shrew in Senegal; Yogue (YOGV), Kasokero (KKOV), and Gossas (GOSV) viruses isolated from bats in Senegal and Uganda; Issyk-Kul virus (IKV) isolated from bats in Kyrgyzstan; and Keterah virus (KTRV) isolated from ticks infesting a bat in Malaysia. The S, M, and L genome segments of each virus were found to encode proteins corresponding to the nucleoprotein, polyglycoprotein, and polymerase protein of CCHFV. However, as observed in Leopards Hill virus (LPHV) and Erve virus (ERVV), polyglycoproteins encoded in the M segment lack sequences encoding the double-membrane-spanning CCHFV NSm protein. Amino acid sequence identities, complement-fixation tests, and phylogenetic analysis indicated that these viruses cluster into three groups comprising KKOV, YOGV, and LPHV from bats of the suborder Yingochiroptera; KTRV, IKV, and GOSV from bats of the suborder Yangochiroptera; and TFAV and ERVV from shrews (Soricomorpha: Soricidae). This reflects clade-specific host and vector associations that extend across the genus.
节肢动物传播的布尼亚病毒中的内罗病毒属包括重要的新出现的人类病原体克里米亚-刚果出血热病毒（CCHFV），以及内罗毕绵羊病病毒和许多其他从哺乳动物、鸟类和蜱中分离出的描述较少的病毒。在此，我们报告了六种内罗病毒的基因组序列分析：从塞内加尔的一只鼩鼱中分离出的蒂亚福拉病毒（TFAV）；从塞内加尔和乌干达的蝙蝠中分离出的约格病毒（YOGV）、卡索克罗病毒（KKOV）和戈萨斯病毒（GOSV）；从吉尔吉斯斯坦的蝙蝠中分离出的伊塞克湖病毒（IKV）；以及从马来西亚一只受蜱侵扰的蝙蝠中分离出的凯特拉病毒（KTRV）。发现每种病毒的S、M和L基因组片段编码的蛋白质分别对应于CCHFV的核蛋白、多聚糖蛋白和聚合酶蛋白。然而，正如在豹山病毒（LPHV）和埃尔夫病毒（ERVV）中观察到的那样，M片段中编码的多聚糖蛋白缺乏编码CCHFV双跨膜NSm蛋白的序列。氨基酸序列同一性、补体结合试验和系统发育分析表明，这些病毒聚为三组：包括来自阴翼手亚目蝙蝠的KKOV、YOGV和LPHV；来自阳翼手亚目蝙蝠的KTRV、IKV和GOSV；以及来自鼩鼱（鼩形目：鼩鼱科）的TFAV和ERVV。这反映了跨该属的特定进化枝宿主和载体关联。
