Araújo Estela R, Sponchiado Mariana, Pugliesi Guilherme, Van Hoeck Veerle, Mesquita Fernando S, Membrive Claudia M B, Binelli Mario
Reprod Fertil Dev. 2014 Apr 21. doi: 10.1071/RD14178.
In cattle, pro-oestrous oestradiol and dioestrous progesterone concentrations modulate endometrial gene expression and fertility. The aim was to compare the effects of different periovulatory endocrine profiles on the expression of progesterone receptor (PGR), oestrogen receptor 2 (ESR2), oxytocin receptor (OXTR), member C4 of aldo-keto reductase family 1 (AKR1C4), lipoprotein lipase (LPL), solute carrier family 2, member 1 (SLC2A1) and serpin peptidase inhibitor, clade A member 14 (SERPINA14): (1) between uterine horns ipsi- and contralateral to the corpus luteum (CL), (2) between regions of the ipsilateral horn and (3) in the vagina. Endometrium and vagina tissue samples were collected from cows that ovulated a larger (large follicle-large CL, LF-LCL; n=6) or smaller follicle (small follicle-small CL, SF-SCL; n=6) 7 days after oestrus. Cows in the LF-LCL group had a greater abundance of transcripts encoding ESR2, AKR1C4, LPL, SLC2A1 and SERPINA14, but a reduced expression of PGR and OXTR in the endometrium versus the SF-SCL group (PPGR and OXTR was greater in the contralateral compared with the ipsilateral horn (PPGR, ESR2, LPL, SLC2A1 and SERPINA14 (P<0.05). Different periovulatory endocrine profiles, i.e. LF-LCL or SF-SCL, did not influence gene expression in the vagina and had no interaction with inter- or intra-uterine horn gene expression. In conclusion, inter- and intra-uterine horn variations in gene expression indicate that the expression of specific genes in the bovine reproductive tract is location dependent. However, spatial distribution of transcripts was not influenced by distinct periovulatory sex-steroid environments.
在牛中，发情前期的雌二醇和发情后期的孕酮浓度可调节子宫内膜基因表达和生育力。本研究旨在比较不同围排卵期内分泌特征对孕酮受体（PGR）、雌激素受体2（ESR2）、催产素受体（OXTR）、醛酮还原酶家族1成员C4（AKR1C4）、脂蛋白脂肪酶（LPL）、溶质载体家族2成员1（SLC2A1）和丝氨酸蛋白酶抑制剂A家族成员14（SERPINA14）表达的影响：（1）黄体同侧和对侧子宫角之间；（2）同侧子宫角不同区域之间；（3）阴道内。在发情后7天，从排卵较大卵泡（大卵泡-大黄体，LF-LCL；n=6）或较小卵泡（小卵泡-小黄体，SF-SCL；n=6）的母牛中采集子宫内膜和阴道组织样本。与SF-SCL组相比，LF-LCL组母牛子宫内膜中编码ESR2、AKR1C4、LPL、SLC2A1和SERPINA14的转录本丰度更高，但PGR和OXTR的表达降低（P<0.05）。PGR和OXTR在对侧子宫角的表达高于同侧子宫角（P<0.05）。不同围排卵期内分泌特征，即LF-LCL或SF-SCL，不影响阴道中的基因表达，也不与子宫角间或子宫角内的基因表达相互作用。总之，子宫角间和子宫角内基因表达的差异表明，牛生殖道中特定基因的表达具有位置依赖性。然而，转录本的空间分布不受不同围排卵期性类固醇环境的影响。
