埃及肥胖个体中瘦素基因四核苷酸重复多态性

Leptin gene tetranucleotide repeat polymorphism in obese individuals in Egypt.

作者信息

Elbaz Rizk, Dawood Nahed, Mostafa Hala, Zaki Somaia, Wafa Alaa, Settin Ahmad

机构信息

Genetics Unit, Faculty of Medicine, Mansoura University.

Zoology Department, Faculty of Science, Tanta University.

出版信息

Int J Health Sci (Qassim). 2015 Jan;9(1):63-71.

PMID:25901134

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4394940/

Abstract

BACKGROUND

Leptin is a peptide hormone secreted by the adipose tissue. Genetic mutations of the leptin gene were reported to cause severe obesity.

OBJECTIVES

This study was undertaken to investigate the association of the polymorphic tetranucleotide repeat locus 3' UTR of leptin gene with obesity in Egyptian cases.

SUBJECTS AND METHODS

This study has included 120 subjects affected with obesity 57 of them were consistent with the diagnosis of metabolic syndrome (MS) while the rest (63) had simple obesity. These cases were compared to 83 normal weight healthy controls. All participants were subjected to an estimation of their body mass index (BMI), waist hip ratio (WHR), serum as well as characterization of leptin gene tetranucleotide repeat (TTTC)n polymorphism by PCR technique.

RESULTS

Thirteen different alleles were identified in all cases of obesity versus only 5 alleles in normal controls. The most frequent allele was the 154 bp allele (57.5% in all cases of obesity vs. 92.2% in controls). Total cases with obesity showed a significantly higher carriage rate of class II alleles (I/II + II/II genotypes) compared to healthy controls (48.3% vs. 6.0%, OR=14.6, 95% CI=5.5-38.6, p=<0.0001). This was more apparent in the group with simple obesity (52.3% vs. 6.0%, OR=17.2, 95% CI=6.1-48.1, p=<0.0001) than in MS cases (43.9 % vs. 6.0 %, OR =12.19, 95% CI=4.9-30.4, p=< 0.0001). Interestingly, cases with MS did not differ from those with simple obesity regarding their class I or II allele frequencies (p> 0.05). Although serum lipids were significantly higher in obese cases compared to controls, no difference was found among obese cases with different leptin gene class genotypes (p> 0.05).

CONCLUSIONS

Tetranucleotide repeat (TTTC)n polymorphism in the 3' UTR of the human leptin gene was associated with obesity in Egyptian obese cases showing higher class II allele carriage rate. However, the lipoprotein levels were not affected by this polymorphism.

摘要

背景

瘦素是一种由脂肪组织分泌的肽类激素。据报道，瘦素基因的基因突变会导致严重肥胖。

目的

本研究旨在调查埃及肥胖患者中瘦素基因3'非翻译区多态性四核苷酸重复序列位点与肥胖的相关性。

对象与方法

本研究纳入了120例肥胖患者，其中57例符合代谢综合征（MS）的诊断，其余63例为单纯性肥胖。将这些病例与83名正常体重的健康对照者进行比较。所有参与者均接受体重指数（BMI）、腰臀比（WHR）、血清的评估，并通过聚合酶链反应（PCR）技术对瘦素基因四核苷酸重复序列（TTTC）n多态性进行特征分析。

结果

在所有肥胖病例中鉴定出13种不同的等位基因，而正常对照组中只有5种等位基因。最常见的等位基因是154 bp等位基因（在所有肥胖病例中占57.5%，在对照组中占92.2%）。与健康对照组相比，肥胖患者中II类等位基因（I/II + II/II基因型）的携带率显著更高（48.3%对6.0%，比值比=14.6，95%置信区间=5.5 - 38.6，p<0.0001）。这在单纯性肥胖组中更为明显（52.3%对