Mahmoodi Narges, Motamed Nasrin, Paylakhi Seyed Hassan, O Mahmoodi Nosrat
Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran. ; School of Biology, University College of Science, University of Tehran, Tehran, Iran.
School of Biology, Damghan University, Damghan, Iran.
Iran J Pharm Res. 2015 Spring;14(2):521-30.
The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced™ in 1:2 ratio (one part silybin bound to two parts phosphatidylcholine) on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium (not serum deprivation). The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells.
多酚水飞蓟宾具有抗氧化和抗癌特性。一些多酚（黄酮类和萜类）的生物利用度较差，可通过将它们与磷脂酰胆碱结合（植物药技术）来提高。许多研究集中在最常见的植物药水飞蓟宾 - 磷脂酰胆碱上，特别是其肝脏保护作用。然而，近年来，也进行了研究以确定其抗癌作用。鉴于血清饥饿不应用于非专注于细胞周期停滞的研究，我们研究了来自Advanced™水飞蓟宾的1:2比例（一份水飞蓟宾与两份磷脂酰胆碱结合）的水飞蓟宾 - 磷脂酰胆碱对在完全培养基（非血清剥夺）中培养的SKBR3乳腺癌细胞系上HER2基因表达的影响。结果与我们之前关于水飞蓟宾对SKBR3细胞上HER2表达的研究进行了比较。使用MTT试验确定细胞处理的浓度，并通过实时RT-PCR定义基因表达。结果显示水飞蓟宾、水飞蓟宾 - 磷脂酰胆碱对SKBR3细胞具有显著的浓度和时间依赖性细胞生长抑制作用以及HER2下调。与相同浓度的水飞蓟宾对SKBR3细胞的作用相比，水飞蓟宾 - 磷脂酰胆碱浓度对细胞生长具有更大的抑制作用和HER2下调作用。
