Shieh Peyton, Dien Vivian T, Beahm Brendan J, Castellano Joseph M, Wyss-Coray Tony, Bertozzi Carolyn R
∥Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, United States.
J Am Chem Soc. 2015 Jun 10;137(22):7145-51. doi: 10.1021/jacs.5b02383. Epub 2015 Jun 2.
Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices.
涵盖可见光谱的荧光生物正交智能探针将能够灵敏地可视化生物系统中代谢标记的分子。在此，我们基于光致电子转移原理提出一种统一设计，以获得一组高荧光叠氮化物探针，这些探针通过点击化学转化为相应的三唑而被激活。这些被称为CalFluors的探针具有从绿色到远红波长范围内的发射最大值，并能在无需洗涤的条件下实现灵敏的生物分子检测。我们使用CalFluor探针在细胞、发育中的斑马鱼和小鼠脑组织切片中对各种炔烃标记的生物分子（聚糖、DNA、RNA和蛋白质）进行成像。
