College of Chemistry and Molecular Engineering, Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871, P. R. China.
Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, P. R. China.
Angew Chem Int Ed Engl. 2022 Oct 24;61(43):e202208707. doi: 10.1002/anie.202208707. Epub 2022 Sep 6.
We report here the development of clickable and highly near-infrared (NIR) fluorescent lanthanide (Ln) complexes for bioorthogonal labeling of biomolecules. These azide- or alkyne-functionalized Ln complexes are hydrophilic and fluorogenic, exhibiting a strong increase of NIR fluorescence upon conjugation with biomolecules. Metabolic labeling of biomolecules with azide or alkyne, followed by click labeling with the Ln complexes, enables NIR fluorescence (NIRF) imaging of DNA, RNA, proteins, and glycans in cells. Furthermore, multicolor imaging is performed by combining click-labeling with the Ln complexes and immunostaining. In addition, the Ln complexes is compatible with click-expansion microscopy (click-ExM), which enables high-resolution NIRF imaging of cellular glycoproteins. Finally, the Ln complexes can be used for time-of-flight secondary-ion mass spectrometry (ToF-SIMS) imaging, thus achieving the first example of dual-modal imaging combining NIRF and SIMS microscopies.
我们在此报告了可点击的和高近红外（NIR）荧光镧系（Ln）配合物的发展，用于生物正交标记生物分子。这些叠氮化物或炔烃功能化的 Ln 配合物具有亲水性和荧光性，在与生物分子缀合后表现出 NIR 荧光的强烈增加。用叠氮化物或炔烃对生物分子进行代谢标记，然后用 Ln 配合物进行点击标记，可实现 DNA、RNA、蛋白质和聚糖在细胞中的 NIR 荧光（NIRF）成像。此外，通过将点击标记与 Ln 配合物和免疫染色相结合，可以进行多色成像。此外，Ln 配合物与点击扩展显微镜（click-ExM）兼容，可实现细胞糖蛋白的高分辨率 NIRF 成像。最后，Ln 配合物可用于飞行时间二次离子质谱（ToF-SIMS）成像，从而实现了将 NIRF 和 SIMS 显微镜相结合的双模式成像的首例。
