Luo Robert F, Curry Cheyenne, Taylor Nathan, Budvytiene Indre, Banaei Niaz
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA.
J Clin Microbiol. 2015 Jul;53(7):2337-9. doi: 10.1128/JCM.00132-15. Epub 2015 Apr 22.
By targeting the erm(41) and rrl genes in the Mycobacterium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed 95% (38/40) concordance with nucleic acid testing and 95% (37/39) concordance with phenotypic testing. This assay provides a simple and rapid alternative to extended incubation or erm(41) sequencing.
通过靶向脓肿分枝杆菌组中的erm(41)和rrl基因,一种用于检测克拉霉素耐药性的多重实时PCR检测方法与核酸检测的一致性为95%(38/40),与表型检测的一致性为95%(37/39)。该检测方法为延长培养或erm(41)测序提供了一种简单快速的替代方法。