Chang Che-Feng, Cai Li, Wang Jian
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Bldg 370B, Baltimore, MD, 21205, USA.
Transl Stroke Res. 2015 Oct;6(5):384-9. doi: 10.1007/s12975-015-0399-5. Epub 2015 Apr 25.
For years, strategies have been proposed to improve translational success in stroke research by improving the quality of animal studies. However, articles that report preclinical intracerebral hemorrhage (ICH) studies continue to lack adequate qualitative and quantitative descriptions of fresh brain tissue collection. They also tend to lack transparency about animal model quality. We conducted a systematic review of 82 ICH research articles to determine the level of detail reported for brain tissue collection. We found that only 24 (29 %) reported the volume, weight, or thickness of tissue collected and a specific description of the anatomical location. Thus, up to 71 % of preclinical ICH research articles did not properly define how fresh specimens were collected for biochemical measurements. Such omissions may impede reproducibility of results between laboratories. Although existing criteria have improved the quality of preclinical stroke studies, ICH researchers need to identify specific guidelines and strategies to avoid pitfalls, minimize bias, and increase reproducibility in this field.
多年来，人们一直提出各种策略，试图通过提高动物研究的质量来提升中风研究中的转化成功率。然而，报道临床前脑出血（ICH）研究的文章仍然缺乏对新鲜脑组织采集的充分定性和定量描述。它们往往也缺乏关于动物模型质量的透明度。我们对82篇ICH研究文章进行了系统综述，以确定脑组织采集所报告的详细程度。我们发现，只有24篇（29%）报告了所采集组织的体积、重量或厚度以及解剖位置的具体描述。因此，高达71%的临床前ICH研究文章没有恰当地定义如何采集新鲜标本用于生化测量。这些遗漏可能会阻碍不同实验室之间结果的可重复性。尽管现有标准已经提高了临床前中风研究的质量，但ICH研究人员需要确定具体的指南和策略，以避免陷阱、尽量减少偏差并提高该领域的可重复性。
