大鼠脑肿瘤的光谱和寿命域测量。
Spectral and lifetime domain measurements of rat brain tumors.
作者信息
Haidar D Abi, Leh B, Zanello M, Siebert R
机构信息
Laboratoire IMNC, UMR 8165, F-91405 Orsay, France ; Université Paris 7, F-75012 Paris, France.
Laboratoire IMNC, UMR 8165, F-91405 Orsay, France.
出版信息
Biomed Opt Express. 2015 Mar 11;6(4):1219-33. doi: 10.1364/BOE.6.001219. eCollection 2015 Apr 1.
Abstract
During glioblastoma surgery, delineation of the brain tumor margins is difficult because the infiltrated and normal tissues have the same visual appearance. We use a fiber-optical fluorescence probe for spectroscopic and time domain measurements to assist surgeon in differentiating the healthy and the infiltrated tissues. First study was performed on rats that were previously injected with tumorous cells. Measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumor brain slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analyzed. The study aimed at defining an optical index that can act as an indicator for discriminating healthy from tumorous tissue.
摘要
在胶质母细胞瘤手术中，由于浸润组织和正常组织外观相同，很难勾勒出脑肿瘤的边缘。我们使用一种光纤荧光探针进行光谱和时域测量，以协助外科医生区分健康组织和浸润组织。首次研究是在先前注射了肿瘤细胞的大鼠身上进行的。对新鲜和固定的大鼠肿瘤脑切片进行了内源性组织荧光测量。分析了光谱特征、荧光氧化还原比率和荧光寿命测量结果。该研究旨在确定一种光学指标，作为区分健康组织和肿瘤组织的指标。
相似文献
1
Spectral and lifetime domain measurements of rat brain tumors.大鼠脑肿瘤的光谱和寿命域测量。
Biomed Opt Express. 2015 Mar 11;6(4):1219-33. doi: 10.1364/BOE.6.001219. eCollection 2015 Apr 1.
2
Fluorescence lifetime spectroscopy of glioblastoma multiforme.多形性胶质母细胞瘤的荧光寿命光谱学
Photochem Photobiol. 2004 Jul-Aug;80:98-103. doi: 10.1562/2003-12-09-RA-023.1.
3
Multimodal imaging to explore endogenous fluorescence of fresh and fixed human healthy and tumor brain tissues.多模态成像技术探索新鲜和固定的人脑组织中内源性荧光的特性。
J Biophotonics. 2019 Mar;12(3):e201800178. doi: 10.1002/jbio.201800178. Epub 2018 Oct 23.
4
Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe.使用荧光寿命成像光纤探头对胃肠道肿瘤活检样本进行描绘。
J Biophotonics. 2024 Jul 16:e202400122. doi: 10.1002/jbio.202400122.
5
Spectral complexity of 5-ALA induced PpIX fluorescence in guided surgery: a clinical study towards the discrimination of healthy tissue and margin boundaries in high and low grade gliomas.5-氨基乙酰丙酸诱导的原卟啉IX荧光在引导手术中的光谱复杂性：一项关于区分高级别和低级别胶质瘤中健康组织与边缘边界的临床研究。
Biomed Opt Express. 2019 Apr 18;10(5):2478-2492. doi: 10.1364/BOE.10.002478. eCollection 2019 May 1.
6
detection of murine glioblastoma through Raman and reflectance fiber-probe spectroscopies.通过拉曼光谱和反射光纤探针光谱检测小鼠胶质母细胞瘤。
Neurophotonics. 2020 Oct;7(4):045010. doi: 10.1117/1.NPh.7.4.045010. Epub 2020 Dec 1.
7
Multispectral Depth-Resolved Fluorescence Lifetime Spectroscopy Using SPAD Array Detectors and Fiber Probes.使用单光子雪崩二极管（SPAD）阵列探测器和光纤探头的多光谱深度分辨荧光寿命光谱学
Sensors (Basel). 2019 Jun 13;19(12):2678. doi: 10.3390/s19122678.
8
Comparative Study Between a Customized Bimodal Endoscope and a Benchtop Microscope for Quantitative Tissue Diagnosis.定制双模态内窥镜与台式显微镜用于定量组织诊断的比较研究
Front Oncol. 2022 May 12;12:881331. doi: 10.3389/fonc.2022.881331. eCollection 2022.
9
Diagnostic potential of autofluorescence for an assisted intraoperative delineation of glioblastoma resection margins.自体荧光在辅助术中确定胶质母细胞瘤切除边缘方面的诊断潜力。
Photochem Photobiol. 2003 Mar;77(3):309-18. doi: 10.1562/0031-8655(2003)077<0309:dpoafa>2.0.co;2.
10
Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS).基于扫描多光谱荧光寿命光谱（ms-TRFS）的实时组织表征技术。
Biomed Opt Express. 2015 Feb 25;6(3):987-1002. doi: 10.1364/BOE.6.000987. eCollection 2015 Mar 1.
引用本文的文献
1
Proof of concept study on differentiating metastatic brain samples by their originating organ using multimodal autofluorescence spectroscopy.使用多模态自发荧光光谱法根据转移脑样本的起源器官对其进行区分的概念验证研究。
Sci Rep. 2025 Apr 3;15(1):11476. doi: 10.1038/s41598-025-92366-1.
2
5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review.5-氨基乙酰丙酸诱导的原卟啉IX荧光光谱在神经外科中的应用综述
Front Neurosci. 2024 Jan 29;18:1310282. doi: 10.3389/fnins.2024.1310282. eCollection 2024.
3
Intraoperative microscopic autofluorescence detection and characterization in brain tumors using stimulated Raman histology and two-photon fluorescence.使用受激拉曼组织学和双光子荧光技术对脑肿瘤进行术中显微自发荧光检测与表征
Front Oncol. 2023 May 10;13:1146031. doi: 10.3389/fonc.2023.1146031. eCollection 2023.
4
Comparative Study Between a Customized Bimodal Endoscope and a Benchtop Microscope for Quantitative Tissue Diagnosis.定制双模态内窥镜与台式显微镜用于定量组织诊断的比较研究
Front Oncol. 2022 May 12;12:881331. doi: 10.3389/fonc.2022.881331. eCollection 2022.
5
Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors.脑肿瘤的无标记宏观荧光寿命成像
Front Oncol. 2021 May 24;11:666059. doi: 10.3389/fonc.2021.666059. eCollection 2021.
6
Molecular changes tracking through multiscale fluorescence microscopy differentiate Meningioma grades and non-tumoral brain tissues.通过多尺度荧光显微镜追踪分子变化可区分脑膜瘤分级和非肿瘤性脑组织。
Sci Rep. 2021 Feb 15;11(1):3816. doi: 10.1038/s41598-020-78678-4.
7
Optical Signatures Derived From Deep UV to NIR Excitation Discriminates Healthy Samples From Low and High Grades Glioma.基于深紫外到近红外激发的光学特征可区分健康样本与低级别和高级别胶质瘤。
Sci Rep. 2019 Jun 19;9(1):8786. doi: 10.1038/s41598-019-45181-4.
8
Spectral complexity of 5-ALA induced PpIX fluorescence in guided surgery: a clinical study towards the discrimination of healthy tissue and margin boundaries in high and low grade gliomas.5-氨基乙酰丙酸诱导的原卟啉IX荧光在引导手术中的光谱复杂性：一项关于区分高级别和低级别胶质瘤中健康组织与边缘边界的临床研究。
Biomed Opt Express. 2019 Apr 18;10(5):2478-2492. doi: 10.1364/BOE.10.002478. eCollection 2019 May 1.
9
Real-time Brain Tumor imaging with endogenous fluorophores: a diagnosis proof-of-concept study on fresh human samples.实时脑肿瘤成像的内源性荧光探针：新鲜人体样本的诊断概念验证研究。
Sci Rep. 2018 Oct 5;8(1):14888. doi: 10.1038/s41598-018-33134-2.
10
The Impact of Compressed Femtosecond Laser Pulse Durations on Neuronal Tissue Used for Two-Photon Excitation Through an Endoscope.压缩飞秒激光脉冲持续时间对经内窥镜用于双光子激发的神经元组织的影响。
Sci Rep. 2018 Jul 24;8(1):11124. doi: 10.1038/s41598-018-29404-8.
本文引用的文献
1
Neurosurgical oncology: advances in operative technologies and adjuncts.神经外科肿瘤学：手术技术与辅助手段的进展
J Neurooncol. 2014 Sep;119(3):451-63. doi: 10.1007/s11060-014-1493-3. Epub 2014 Jun 27.
2
Neurosurgical confocal endomicroscopy: A review of contrast agents, confocal systems, and future imaging modalities.神经外科共聚焦内镜检查：造影剂、共聚焦系统及未来成像模式综述
Surg Neurol Int. 2014 Apr 28;5:60. doi: 10.4103/2152-7806.131638. eCollection 2014.
3
Fluorescence spectroscopy as a highly potential single-entity tool to identify chromophores and fluorophores: study on neoplastic human brain lesions.荧光光谱学作为一种极具潜力的单一实体工具，可用于识别生色团和荧光团：对肿瘤性人脑病变的研究。
J Biomed Opt. 2013 Jun;18(6):067002. doi: 10.1117/1.JBO.18.6.067002.
4
Optical phantoms with variable properties and geometries for diffuse and fluorescence optical spectroscopy.具有可变性质和几何形状的光学模型，用于漫反射和荧光光谱学。
J Biomed Opt. 2012 Oct;17(10):108001. doi: 10.1117/1.JBO.17.10.108001.
5
Intraoperative confocal microscopy in the visualization of 5-aminolevulinic acid fluorescence in low-grade gliomas.术中共聚焦显微镜在低级别胶质瘤中可视化 5-氨基酮戊酸荧光。
J Neurosurg. 2011 Oct;115(4):740-8. doi: 10.3171/2011.6.JNS11252. Epub 2011 Jul 15.
6
Compact point-detection fluorescence spectroscopy system for quantifying intrinsic fluorescence redox ratio in brain cancer diagnostics.用于定量脑癌诊断中固有荧光氧化还原比的紧凑型点检测荧光光谱系统。
J Biomed Opt. 2011 Mar;16(3):037004. doi: 10.1117/1.3558840.
7
Fluorescence lifetime spectroscopy for guided therapy of brain tumors.荧光寿命光谱学在脑肿瘤引导治疗中的应用。
Neuroimage. 2011 Jan;54 Suppl 1(Suppl 1):S125-35. doi: 10.1016/j.neuroimage.2010.11.001. Epub 2010 Nov 3.
8
Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery.荧光寿命成像显微镜在脑肿瘤图像引导手术中的应用。
J Biomed Opt. 2010 Sep-Oct;15(5):056022. doi: 10.1117/1.3486612.
9
Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy.应用时间分辨荧光光谱术对原发性脑肿瘤进行术中描绘。
J Biomed Opt. 2010 Mar-Apr;15(2):027008. doi: 10.1117/1.3374049.
10
Longitudinal optical imaging of tumor metabolism and hemodynamics.肿瘤代谢和血液动力学的纵向光学成像。
J Biomed Opt. 2010 Jan-Feb;15(1):011112. doi: 10.1117/1.3285584.
Zotero 插件