IMNC Laboratory, UMR 8165-CNRS/IN2P3, Paris-Saclay university, 91405, Orsay, France.
Neurosurgery Department, Sainte-Anne Hospital, Paris, France.
Sci Rep. 2018 Oct 5;8(1):14888. doi: 10.1038/s41598-018-33134-2.
The primary line of therapy for high-grade brain tumor is surgical resection, however, identifying tumor margins in vivo remains a major challenge. Despite the progress in computer-assisted imaging techniques, biopsy analysis remains the standard diagnostic tool when it comes to delineating tumor margins. Our group aims to answer this challenge by exploiting optical imaging of endogenous fluorescence in order to provide a reliable and reproducible diagnosis close to neuropathology. In this study, we first establish the ability of two-photon microscopy (TPM) to discriminate normal brain tissue from glioblastomas and brain metastasis using the endogenous fluorescence response of fresh human brain sample. Two-photon fluorescence images were compared to gold standard neuropathology. "Blind" diagnosis realized by a neuropathologist on a group of TPM images show a good sensitivity, 100%, and specificity, 50% to discriminate non tumoral brain tissue versus glioblastoma or brain metastasis. Quantitative analysis on spectral and fluorescence lifetime measurements resulted in building a scoring system to discriminate brain tissue samples.
高级别脑肿瘤的主要治疗方法是手术切除，然而，在体内识别肿瘤边界仍然是一个主要挑战。尽管计算机辅助成像技术取得了进展，但活检分析仍然是描绘肿瘤边界的标准诊断工具。我们的研究小组旨在通过利用内源性荧光的光学成像来应对这一挑战，以便提供接近神经病理学的可靠和可重复的诊断。在这项研究中，我们首先使用新鲜人脑样本的内源性荧光反应，建立了双光子显微镜（TPM）区分正常脑组织与胶质母细胞瘤和脑转移的能力。将双光子荧光图像与金标准神经病理学进行比较。神经病理学家对一组 TPM 图像进行的“盲目”诊断显示出良好的敏感性，100%，特异性，50%，可区分非肿瘤性脑组织与胶质母细胞瘤或脑转移。对光谱和荧光寿命测量的定量分析导致建立了一个评分系统来区分脑组织样本。
