一种用于无标记检测淋巴瘤癌细胞的新策略。
A new strategy for label-free detection of lymphoma cancer cells.
作者信息
Martucci Nicola M, Rea Ilaria, Ruggiero Immacolata, Terracciano Monica, De Stefano Luca, Migliaccio Nunzia, Palmieri Camillo, Scala Giuseppe, Arcari Paolo, Rendina Ivo, Lamberti Annalisa
机构信息
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131 Naples, Italy.
出版信息
Biomed Opt Express. 2015 Mar 19;6(4):1353-62. doi: 10.1364/BOE.6.001353. eCollection 2015 Apr 1.
In this paper, a new strategy for highly selective and sensitive direct detection of lymphoma cells by exploiting the interaction between a peptide and its B-cell receptor, has been evaluated. In particular, an idiotype peptide, able to specifically bind the B-cell receptor of A20 cells in mice engrafted with A20 lymphoma, has been used as molecular probe. The new detection technique has been demonstrated on a planar crystalline silicon chip. Coverage of 85% of silicon surface and detection efficiency of 8.5 × 10(-3) cells/μm(2) were obtained. The recognition strategy promises to extend its application in studying the interaction between ligands and their cell-surface receptors.
在本文中，一种通过利用肽与其B细胞受体之间的相互作用来高选择性和灵敏地直接检测淋巴瘤细胞的新策略已得到评估。具体而言，一种能够特异性结合移植有A20淋巴瘤的小鼠体内A20细胞的B细胞受体的独特型肽已被用作分子探针。这种新的检测技术已在平面晶体硅芯片上得到验证。获得了85%的硅表面覆盖率和8.5×10(-3)个细胞/μm(2)的检测效率。该识别策略有望在研究配体与其细胞表面受体之间的相互作用方面拓展其应用。
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