Romano Silvia, Lamberti Annalisa, Masullo Mariorosario, Penzo Erika, Cabrini Stefano, Rendina Ivo, Mocella Vito
Institute for Microelectronics and Microsystems-Unit of Naples-National Council of Research, Via Pietro Castellino, 80131 Naples, Italy.
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Napoli, Italy.
Materials (Basel). 2018 Mar 30;11(4):526. doi: 10.3390/ma11040526.
A novel optical label-free bio-sensing platform based on a new class of resonances supported in a photonic crystal metasurface is reported herein. Molecular binding is detected as a shift in the resonant wavelength of the bound states in the continuum of radiation modes. The new configuration is applied to the recognition of the interaction between protein p53 and its protein regulatory partner murine double minute 2 (MDM2). A detection limit of 66 nM for the protein p53 is found. The device provides an excellent interrogation stability and loss-free operation, requires minimal optical interrogation equipment and can be easily optimized to work in a wide wavelength range.
本文报道了一种基于光子晶体超表面中支持的新型共振的新型无光学标记生物传感平台。分子结合被检测为辐射模式连续体中束缚态共振波长的变化。这种新配置被应用于识别蛋白质p53与其蛋白质调节伙伴小鼠双微体2（MDM2）之间的相互作用。发现p53蛋白的检测限为66 nM。该装置具有出色的检测稳定性和无损耗操作，所需的光学检测设备最少，并且可以轻松优化以在很宽的波长范围内工作。
