木棉（锦葵科）EST-SSR标记的开发与特征分析
Development and characterization of EST-SSR markers in Bombax ceiba (Malvaceae).
作者信息
Ju Miao-Miao, Ma Huan-Cheng, Xin Pei-Yao, Zhou Zhi-Li, Tian Bin
机构信息
Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China ; Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
出版信息
Appl Plant Sci. 2015 Apr 3;3(4). doi: 10.3732/apps.1500001. eCollection 2015 Apr.
PREMISE OF THE STUDY
Bombax ceiba (Malvaceae), commonly known as silk cotton tree, is a multipurpose tree species of tropical forests. Novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for the species using transcriptome analysis.
METHODS AND RESULTS
A total of 33 new EST-SSR markers were developed for B. ceiba, of which 13 showed polymorphisms across the 24 individuals from four distant populations tested in the study. The results showed that the number of alleles per polymorphic locus ranged from two to four, and the expected heterozygosity and observed heterozygosity per locus varied from 0.043 to 0.654 and from 0 to 0.609, respectively.
CONCLUSIONS
These newly developed EST-SSR markers can be used in phylogeographic and population genetic studies to investigate the origin of B. ceiba populations. Furthermore, these EST-SSR markers could also greatly promote the development of molecular breeding studies pertaining to silk cotton tree.
研究前提
木棉（锦葵科），通常被称为丝棉树，是热带森林中的一种多用途树种。利用转录组分析为该物种开发并鉴定了新型表达序列标签-简单序列重复（EST-SSR）标记。
方法与结果
为木棉开发了总共33个新的EST-SSR标记，其中13个在研究中测试的来自四个不同种群的24个个体中显示出多态性。结果表明，每个多态性位点的等位基因数量在2到4个之间，每个位点的预期杂合度和观察到的杂合度分别在0.043到0.654和0到0.609之间。
结论
这些新开发的EST-SSR标记可用于系统地理学和种群遗传学研究，以调查木棉种群的起源。此外，这些EST-SSR标记也可极大地促进丝棉树分子育种研究的发展。
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