虎耳草变种（虎耳草科）EST-SSR标记的开发与特征分析
Development and characterization of EST-SSR markers for var. (Saxifragaceae).
作者信息
Magota Kana, Takahashi Daiki, Setoguchi Hiroaki
机构信息
Graduate School of Human and Environmental Studies Kyoto University Yoshida Nihonmatsu-cho, Sakyo-ku Kyoto 606-8501 Japan.
出版信息
Appl Plant Sci. 2019 Jul 9;7(7):e11275. doi: 10.1002/aps3.11275. eCollection 2019 Jul.
PREMISE
(Saxifragaceae) includes several infraspecific taxa that are ecologically and morphologically distinct. To investigate the evolutionary history of phenotypic polymorphisms in this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers for . .
METHODS AND RESULTS
We developed 26 polymorphic markers based on transcriptome data obtained from Illumina HiSeq 2000. Within three populations of . var. , the number of alleles ranged from four to 25, and the levels of observed and expected heterozygosity ranged from 0.200 to 0.847 and from 0.209 to 0.930, respectively. Furthermore, all 26 loci showed transferability for . var. and . var. , and 18 loci were also successfully amplified in . .
CONCLUSIONS
These newly developed EST-SSR markers will prove useful to infer the evolutionary history of . var. and its relatives in population genetic studies.
前提
虎耳草科包含几个在生态和形态上有明显差异的种下分类群。为了研究该物种表型多态性的进化历史，我们开发了用于……的表达序列标签-简单序列重复（EST-SSR）标记。
方法与结果
我们基于从Illumina HiSeq 2000获得的转录组数据开发了26个多态性标记。在……变种的三个种群中，等位基因数量从4到25不等，观察到的杂合度和预期杂合度水平分别从0.200到0.847和从0.209到0.930。此外，所有26个位点在……变种和……变种中都具有可转移性，并且18个位点在……中也成功扩增。
结论
这些新开发的EST-SSR标记将被证明在群体遗传学研究中有助于推断……变种及其近缘种的进化历史。
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