Jung Ho Sang, Lee Taekyung, Kwon Il Keun, Kim Hyoung Seop, Hahn Sei Kwang, Lee Chong Soo
†Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 790-784, Korea.
‡Department of Mechanical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3111, United States.
ACS Appl Mater Interfaces. 2015 May 13;7(18):9598-607. doi: 10.1021/acsami.5b03431. Epub 2015 May 4.
Titanium (Ti) and its alloys with a high mechanical strength and a small diameter can be effectively exploited for minimally invasive dental implantation. Here, we report a multipass caliber-rolled Ti alloy of Ti13Nb13Zr (MPCR-TNZ) with a high mechanical strength and strong fatigue characteristics. For further dental applications, MPCR-TNZ was surface-modified with reduced graphene oxide (RGO) and loaded with osteogenic dexamethasone (Dex) via π-π stacking on the graphitic domain of RGO. The Dex-loaded RGO-MPCR-TNZ (Dex/RGO-MPCR-TNZ) resulted in significantly enhanced growth and differentiation of MC3T3-E1 cells into osteoblasts, which was confirmed by Alizarin red staining, alkaline phosphatase activity test, immunocytochemistry, and real-time PCR. Moreover, we could confirm the feasibility of Dex/RGO-MPCR-TNZ from the implantation test of a prototype of a dental implant to an artificial bone block for clinical dental applications.
钛（Ti）及其具有高机械强度和小直径的合金可有效地用于微创牙科植入。在此，我们报告了一种具有高机械强度和强疲劳特性的多道次口径轧制Ti13Nb13Zr钛合金（MPCR-TNZ）。为了进一步用于牙科应用，MPCR-TNZ用还原氧化石墨烯（RGO）进行表面改性，并通过在RGO的石墨域上的π-π堆积负载成骨地塞米松（Dex）。负载Dex的RGO-MPCR-TNZ（Dex/RGO-MPCR-TNZ）导致MC3T3-E1细胞向成骨细胞的生长和分化显著增强，这通过茜素红染色、碱性磷酸酶活性测试、免疫细胞化学和实时PCR得到证实。此外，我们可以通过将牙科植入物原型植入人工骨块用于临床牙科应用的测试来确认Dex/RGO-MPCR-TNZ的可行性。
