Ongaro A, Campana L G, De Mattei M, Dughiero F, Forzan M, Pellati A, Rossi C R, Sieni E
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
Sarcoma and Melanoma Unit, Veneto Institute of Oncology (IOV-IRCCS), Padova, Italy.
Technol Cancer Res Treat. 2016 Apr;15(2):296-307. doi: 10.1177/1533034615582350. Epub 2015 Apr 24.
Electrochemotherapy (ECT) is a local anticancer treatment based on the combination of chemotherapy and short, tumor-permeabilizing, voltage pulses delivered using needle electrodes or plate electrodes. The application of ECT to large skin surface tumors is time consuming due to technical limitations of currently available voltage applicators. The availability of large pulse applicators with few and more spaced needle electrodes could be useful in the clinic, since they could allow managing large and spread tumors while limiting the duration and the invasiveness of the procedure. In this article, a grid electrode with 2-cm spaced needles has been studied by means of numerical models. The electroporation efficiency has been assessed on human osteosarcoma cell line MG63 cultured in monolayer. The computational results show the distribution of the electric field in a model of the treated tissue. These results are helpful to evaluate the effect of the needle distance on the electric field distribution. Furthermore, the in vitro tests showed that the grid electrode proposed is suitable to electropore, by a single application, a cell culture covering an area of 55 cm(2). In conclusion, our data might represent substantial improvement in ECT in order to achieve a more homogeneous and time-saving treatment, with benefits for patients with cancer.
电化学疗法（ECT）是一种局部抗癌治疗方法，它基于化疗与使用针电极或平板电极施加的短暂、可使肿瘤通透的电压脉冲相结合。由于目前可用的电压施加器存在技术限制，将ECT应用于大面积皮肤表面肿瘤非常耗时。具有较少且间隔较大的针电极的大型脉冲施加器在临床上可能会很有用，因为它们可以在管理大型和扩散性肿瘤的同时，限制手术的持续时间和侵入性。在本文中，通过数值模型研究了一种针间距为2厘米的网格电极。在单层培养的人骨肉瘤细胞系MG63上评估了电穿孔效率。计算结果显示了处理后组织模型中的电场分布。这些结果有助于评估针间距对电场分布的影响。此外，体外测试表明，所提出的网格电极通过单次应用就适合对面积为55平方厘米的细胞培养物进行电穿孔。总之，我们的数据可能代表了ECT的实质性改进，以便实现更均匀且节省时间的治疗，对癌症患者有益。
