Bazzolo Bianca, Sieni Elisabetta, Zamuner Annj, Roso Martina, Russo Teresa, Gloria Antonio, Dettin Monica, Conconi Maria Teresa
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, 35131 Padova, Italy.
Department of Theoretical and Applied Sciences, University of Insubria, via Dunant, 3, 21100 Varese, Italy.
Bioengineering (Basel). 2020 Dec 22;8(1):1. doi: 10.3390/bioengineering8010001.
During anticancer drug development, most compounds selected by in vitro screening are ineffective in in vivo studies and clinical trials due to the unreliability of two-dimensional (2D) in vitro cultures that are unable to mimic the cancer microenvironment. Herein, HCC1954 cell cultures on electrospun polycaprolactone (PCL) were characterized by morphological analysis, cell viability assays, histochemical staining, immunofluorescence, and RT-PCR. Our data showed that electrospun PCL allows the in vitro formation of cultures characterized by mucopolysaccharide production and increased cancer stem cell population. Moreover, PCL-based cultures were less sensitive to doxorubicin and electroporation/bleomycin than those grown on polystyrene plates. Collectively, our data indicate that PCL-based cultures may be promising tools for preclinical studies.
在抗癌药物研发过程中，由于无法模拟癌症微环境的二维（2D）体外培养不可靠，大多数通过体外筛选选出的化合物在体内研究和临床试验中无效。在此，通过形态学分析、细胞活力测定、组织化学染色、免疫荧光和逆转录聚合酶链反应（RT-PCR）对电纺聚己内酯（PCL）上的HCC1954细胞培养物进行了表征。我们的数据表明，电纺PCL能够在体外形成以粘多糖产生和癌症干细胞群体增加为特征的培养物。此外，与在聚苯乙烯平板上生长的培养物相比，基于PCL的培养物对多柔比星和电穿孔/博来霉素的敏感性较低。总体而言，我们的数据表明基于PCL的培养物可能是临床前研究的有前途的工具。
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