NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.
NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.
Biosens Bioelectron. 2015 Sep 15;71:278-285. doi: 10.1016/j.bios.2015.04.026. Epub 2015 Apr 14.
A novel phosphate amperometric nanobiosensor, based on an intimate integration of pyruvate oxidase (PyOx) and its cofactors, thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), with a highly ordered gold nanowires array (AuNWA) has been developed. The successful integration of PyOx and the co-factors, via crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLA), onto the AuNWA was confirmed by cyclic voltammetry and amperometry. The resulting nanobiosensor achieved a detection limit of 0.1 µM, a linear concentration range of 12.5-1000 µM, and a sensitivity of 140.3 µA mM(-1)cm(-2). Notably, the incorporation of the AuNWA reduced the required PyOx concentration by 70-120 fold and the presence of common interferants, such as chloride, sulfate, fluoride, nitrite and nitrate ions did not interfere with phosphate detection. Furthermore, the nanobiosensor demonstrated a very high stability with repeated use over two weeks and was successfully used for the determination of phosphate in water samples with an average recovery of 96.6 ± 4.9%.
一种新型的磷酸安培纳米生物传感器，基于丙酮酸氧化酶（PyOx）及其辅因子，即焦磷酸硫胺素（TPP）和黄素腺嘌呤二核苷酸（FAD）与高度有序的金纳米线阵列（AuNWA）的紧密结合而开发。通过与牛血清白蛋白（BSA）和戊二醛（GLA）交联，成功地将 PyOx 和辅因子整合到 AuNWA 上，这一点通过循环伏安法和安培法得到了证实。所得纳米生物传感器的检测限为 0.1µM，线性浓度范围为 12.5-1000µM，灵敏度为 140.3µA mM(-1)cm(-2)。值得注意的是，AuNWA 的加入将所需的 PyOx 浓度降低了 70-120 倍，并且常见的干扰物，如氯离子、硫酸根离子、氟离子、亚硝酸盐离子和硝酸盐离子，不会干扰磷酸根的检测。此外，该纳米生物传感器具有非常高的稳定性，可重复使用两周以上，并成功用于水样中磷酸盐的测定，平均回收率为 96.6±4.9%。
