Chamnanchanunt Supat, Kuroki Chieri, Desakorn Varunee, Enomoto Mari, Thanachartwet Vipa, Sahassananda Duangjai, Sattabongkot Jetsumon, Jenwithisuk Rachaneeporn, Fucharoen Suthat, Svasti Saovaros, Umemura Tsukuru
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Department of Medical Technology, Graduate School of Health Sciences, Kyushu University, Fukuoka, Japan.
Exp Parasitol. 2015 Aug;155:19-25. doi: 10.1016/j.exppara.2015.04.013. Epub 2015 Apr 22.
Malaria is a common parasitic disease in tropical countries, causing one to two million deaths every year. To establish the new biomarker, we analyzed plasma miRNAs obtained from 19 malaria patients and 19 normal subjects, using reverse transcription-based quantitative polymerase chain reaction (RT-qPCR). The average levels of plasma miR-451 and miR-16 were significantly lower in malaria patients, (8.9-fold; p <0.001 and 10.4-fold; p = 0.01, respectively). The levels of other abundant miRNAs in plasma (miR-223, miR-226-3p) did not change significantly in malaria patients. Our data suggest that plasma miR-451 and miR-16 are relevant biomarkers for malaria infection.
疟疾是热带国家常见的寄生虫病，每年导致100万至200万人死亡。为了建立新的生物标志物，我们使用基于逆转录的定量聚合酶链反应（RT-qPCR）分析了19名疟疾患者和19名正常受试者的血浆miRNA。疟疾患者血浆miR-451和miR-16的平均水平显著降低（分别为8.9倍；p<0.001和10.4倍；p = 0.01）。血浆中其他丰富的miRNA（miR-223、miR-226-3p）水平在疟疾患者中没有显著变化。我们的数据表明，血浆miR-451和miR-16是疟疾感染的相关生物标志物。
