Liu Guangbin, Liu Ruize, Tang Xiaohui, Cao Jianhua, Zhao Shuhong, Yu Mei
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2015 Apr 23;16(5):9152-66. doi: 10.3390/ijms16059152.
Wool is an important material in textile manufacturing. In order to investigate the intrinsic factors that regulate wool follicle cycling and wool fiber properties, Illumina sequencing was performed on wool follicle bulb samples from the middle anagen, catagen and late telogen/early anagen phases. In total, 13,898 genes were identified. KRTs and KRTAPs are the most highly expressed gene families in wool follicle bulb. In addition, 438 and 203 genes were identified to be differentially expressed in wool follicle bulb samples from the middle anagen phase compared to the catagen phase and the samples from the catagen phase compared to the late telogen/early anagen phase, respectively. Finally, our data revealed that two groups of genes presenting distinct expression patterns during the phase transformation may have important roles for wool follicle bulb regression and regeneration. In conclusion, our results demonstrated the gene expression patterns in the wool follicle bulb and add new data towards an understanding of the mechanisms involved in wool fiber growth in sheep.
羊毛是纺织制造业中的一种重要材料。为了研究调节毛囊周期和羊毛纤维特性的内在因素，对处于生长期中期、退行期以及休止期末期/生长期初期的毛囊球样本进行了Illumina测序。总共鉴定出13898个基因。角蛋白（KRTs）和角蛋白关联蛋白（KRTAPs）是毛囊球中表达量最高的基因家族。此外，分别鉴定出438个和203个基因在生长期中期与退行期的毛囊球样本之间以及退行期与休止期末期/生长期初期的毛囊球样本之间存在差异表达。最后，我们的数据表明，两组在阶段转变过程中呈现出不同表达模式的基因可能对毛囊球的退化和再生具有重要作用。总之，我们的结果展示了毛囊球中的基因表达模式，并为理解绵羊羊毛纤维生长所涉及的机制增添了新的数据。
