Kálmán M, Hajós F
First Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Exp Brain Res. 1989;78(1):147-63. doi: 10.1007/BF00230694.
In the first of two papers dealing with the distribution of glial fibrillary acidic protein-(GFAP)-immunoreactive elements in the rat brain, the localization of immunostaining in the forebrain is systematically described. While the limbic cortex was found to contain intensely stained, evenly distributed astrocytes, the neocortex showed clearly stratified GFAP-staining, with substantially less immunoreactivity occurring in the middle layers than in the areas close to the brain surface or the white matter. A remarkably regular staining pattern was observed in the hippocampus and dentate gyrus. The striatum remained unstained in sharp contrast to the pallidum. In the diencephalon, the main thalamic nuclei were poor in GFAP-labelled elements in contrast to the internuclear border zones. In the hypothalamus, nuclei were conspicuous by their GFAP-staining. A consistent differential staining pattern was obtained in the epithalamic structures. The observed distributional pattern of diencephalic GFAP-immunoreactivity is thought to be due to different regional proliferation of the embryonic neuroepithelium of the diencephalon. The uneven distribution of GFAP-immunoreactivity in the forebrain is explained on a mainly developmental basis.
在两篇关于大鼠脑中胶质纤维酸性蛋白（GFAP）免疫反应性成分分布的论文中的第一篇里，系统描述了前脑免疫染色的定位。虽然发现边缘皮质含有染色强烈、分布均匀的星形胶质细胞，但新皮质显示出明显分层的GFAP染色，中层的免疫反应性明显低于靠近脑表面或白质的区域。在海马体和齿状回观察到显著规则的染色模式。与苍白球形成鲜明对比的是，纹状体未染色。在间脑，主要丘脑核中的GFAP标记成分较少，与核间边界区形成对比。在下丘脑，各核因其GFAP染色而明显可见。在上丘脑结构中获得了一致的差异染色模式。间脑GFAP免疫反应性的观察分布模式被认为是由于间脑胚胎神经上皮不同区域的增殖所致。前脑中GFAP免疫反应性的不均匀分布主要从发育角度进行了解释。
