Zarban Asghar, Toroghi Mahsa Mostafavi, Asli Marziye, Jafari Masumeh, Vejdan Morteza, Sharifzadeh Gholamreza
1 Biochemistry Department, Birjand University of Medical Sciences , Birjand, Islamic Republic of Iran .
Breastfeed Med. 2015 May;10(4):214-7. doi: 10.1089/bfm.2014.0143.
After delivery and birth, mothers and neonates are exposed to oxidative stress. The present study examined the effect of supplementation of the diet of breastfeeding mothers with vitamin C and E to improve the antioxidant content of breastmilk and evidence of antioxidant activity in infant urine.
The subjects were 60 healthy lactating breastfeeding mothers and their infants 1-6 months of age. They were randomly allocated to a control group (n=30) consuming a free diet or an experimental group (n=30) consuming a free diet supplemented each day with effervescent tablets of vitamin C (500 mg) and chewable tablets of vitamin E (100 IU). After 30 days, the total antioxidant content of the mothers' breastmilk and evidence of antioxidant activity in the infants' urine were measured by the ferric reducing/antioxidant power assay. The free radical scavenging activity of the urine samples was measured by the α,α-diphenyl-β-picrylhydrazyl method. Differences pre- and postintervention were compared within and between the groups.
Significantly higher levels of antioxidants in the breastmilk (610±295.5 to 716±237.5 μmol/L) and infant urine (43.2±21.8 to 75.0±49.2 μmol/mg creatinine) were observed in the experimental group over the control group (p<0.05). A significant increase in evidence of free radical scavenging in infant urine was observed in the experimental group after 30 days of supplementation by mothers (p<0.05).
Consumption of vitamin C and E supplements appears to have a positive effect on total antioxidant content of breastmilk and evidence of antioxidant activity in infant urine.
分娩和出生后，母亲和新生儿会受到氧化应激的影响。本研究检测了哺乳期母亲饮食中补充维生素C和E对提高母乳抗氧化成分以及婴儿尿液中抗氧化活性证据的影响。
研究对象为60名健康的哺乳期母亲及其1至6个月大的婴儿。她们被随机分为对照组（n = 30），食用自由饮食；或实验组（n = 30），食用自由饮食并每天补充维生素C泡腾片（500毫克）和维生素E咀嚼片（100国际单位）。30天后，通过铁还原/抗氧化能力测定法测量母亲母乳中的总抗氧化成分以及婴儿尿液中抗氧化活性的证据。通过α，α-二苯基-β-苦味酰肼法测量尿液样本的自由基清除活性。比较组内和组间干预前后的差异。
实验组母乳中的抗氧化剂水平（610±295.5至716±237.5微摩尔/升）和婴儿尿液中的抗氧化剂水平（43.2±21.8至75.0±49.2微摩尔/毫克肌酐）显著高于对照组（p<0.05）。母亲补充30天后，实验组婴儿尿液中自由基清除证据显著增加（p<0.05）。
补充维生素C和E似乎对母乳中的总抗氧化成分以及婴儿尿液中的抗氧化活性证据有积极影响。
