Andrés-Barrao Cristina, Lafi Feras F, Alam Intikhab, de Zélicourt Axel, Eida Abdul A, Bokhari Ameerah, Alzubaidy Hanin, Bajic Vladimir B, Hirt Heribert, Saad Maged M
Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Front Microbiol. 2017 Oct 20;8:2023. doi: 10.3389/fmicb.2017.02023. eCollection 2017.
sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant . SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to . The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were identified that potentially contribute to plant growth promotion. The information encoded in the genome of SA187 reveals the characteristics of a dualistic lifestyle of a bacterium that can adapt to different environments and promote the growth of plants. This information provides a better understanding of the mechanisms involved in plant-microbe interaction and could be further exploited to develop SA187 as a biological agent to improve agricultural practices in marginal and arid lands.
sp. SA187是一种从本土沙漠植物根瘤中分离出的内生细菌。SA187能够在根际以及与不同植物物种共生的环境中存活，并能够为（植物）提供非生物胁迫耐受性。SA187的基因组序列是通过使用太平洋生物科学公司（PacBio）的单分子测序技术获得的，平均覆盖度为275倍。SA187的基因组由一条单一的4,429,597 bp染色体组成，平均GC含量为56%，预测有4,347个蛋白质编码DNA序列（CDS）、153个非编码RNA、7个rRNA和84个tRNA。对SA187基因组的功能分析揭示了大量参与营养物质摄取和交换、趋化作用、移动和植物定殖的基因。还发现大量基因参与生存、抗氧化应激防御以及抗菌化合物和毒素的产生。此外，还鉴定出了可能有助于促进植物生长的不同代谢途径。SA187基因组中编码的信息揭示了一种细菌二元生活方式的特征，这种细菌能够适应不同环境并促进植物生长。这些信息有助于更好地理解植物 - 微生物相互作用的机制，并可进一步利用来开发SA187作为一种生物制剂，以改善边际和干旱土地的农业实践。
