Mart'ianov S V, Zhurina M V, Él'-Registan G I, Plakunov V K
Mikrobiologiia. 2015 Jan-Feb;84(1):27-36.
Growth of members of most of the studied genera of gram-positive (Dietzia, Kocuria, and Rhodo- coccus) and gram-negative bacteria (Pseudomonas and Chromobacterium) in biofilms exhibited higher resistance to an translation inhibitor, azithromycin compared to the growth of planktonic cultures of the same strains. Low concentrations of azithromycin were found to stimulate biofilm formation by the studied saprotrophic strains. The rate of synthesis of the polysaccharide matrix component exceeded the rate of cell growth, indicating implementation of the biofilm phenotype under these conditions. It was found that an alkylhydroxybenzene (AHB) compound 4-hexylresorcinol was capable of almost uniform suppression of growth of both planktonic cultures and biofilms of the saprotrophic strains under study. In some cases, combined action ofazithromycin and AHB resulted in an additive inhibitory effect and prevented the stimulation of biofilm growth by subinhibitory azithromycin concentrations. Thus, AHB may be considered a promising antibiofilm agent.
与相同菌株的浮游培养物生长相比，大多数研究的革兰氏阳性菌属（Dietzia、Kocuria和红球菌属）和革兰氏阴性菌属（假单胞菌属和色杆菌属）成员在生物膜中的生长对翻译抑制剂阿奇霉素表现出更高的抗性。发现低浓度的阿奇霉素可刺激所研究的腐生菌株形成生物膜。多糖基质成分的合成速率超过细胞生长速率，表明在这些条件下生物膜表型得以实现。发现一种烷基羟基苯（AHB）化合物4-己基间苯二酚能够几乎均匀地抑制所研究腐生菌株的浮游培养物和生物膜的生长。在某些情况下，阿奇霉素和AHB的联合作用产生相加抑制作用，并防止亚抑制浓度的阿奇霉素刺激生物膜生长。因此，AHB可被认为是一种有前景的抗生物膜剂。
