Mart'yanov Sergey Vladislavovich, Botchkova Ekaterina Alexandrovna, Plakunov Vladimir Konstantinovich, Gannesen Andrei Vladislavovich
Laboratory of Viability of Microorganisms, Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences, 117312 Moscow, Russia.
Laboratory of Microbiology of Anthropogenic Habitats, Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences, 117312 Moscow, Russia.
Microorganisms. 2021 Apr 13;9(4):820. doi: 10.3390/microorganisms9040820.
The effect of norepinephrine ("NE") on Gram-negative bacteria is well characterized; however, little is known about the impact of NE on cutaneous Gram-positive skin residents, especially staphylococci. In this study, the impact of NE on monospecies and dual-species biofilms of and model strains was investigated for the first time. Biofilms were grown in two different models (on polytetrafluoroethylene ("PTFE") cubes and glass microfiber filters ("GMFFs")) and additionally kinetic measurements of bacterial growth was performed. We have shown that NE can affect the biofilm formation of both species with a strong dependence on aerobic or anaerobic culture conditions in different models. It was shown that suppresses growth in dual-species biofilms and that NE can accelerate this process, contributing to the competitive behavior of staphylococci.
去甲肾上腺素（“NE”）对革兰氏阴性菌的作用已得到充分表征；然而，关于NE对皮肤革兰氏阳性常驻菌，尤其是葡萄球菌的影响却知之甚少。在本研究中，首次研究了NE对金黄色葡萄球菌和表皮葡萄球菌模型菌株的单物种和双物种生物膜的影响。生物膜在两种不同模型（聚四氟乙烯（“PTFE”）立方体和玻璃微纤维滤器（“GMFFs”））中生长，此外还进行了细菌生长的动力学测量。我们已经表明，NE可以影响两种菌的生物膜形成，且在不同模型中强烈依赖于需氧或厌氧培养条件。结果表明，表皮葡萄球菌在双物种生物膜中抑制金黄色葡萄球菌的生长，并且NE可以加速这一过程，促进葡萄球菌的竞争行为。
