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[粘质沙雷氏菌的色素沉着及灵菌红素的光谱特性]

[Pigmentation of Serratia marcescens and spectral properties of prodigiosin].

作者信息

Andreeva I N, Ogorodnikova T I

出版信息

Mikrobiologiia. 2015 Jan-Feb;84(1):43-9.

Abstract

Pigmentation of Serratia marcescens depends on the composition of the cultivation medium. The cultures grown on glycerol-peptone medium and on the medium with acetate are red and yellow (yellowish orange), respectively, with the color depending on the ambient pH. S. marcescens cells growth on glycerol-peptone medium (visually of red color) contain two forms of prodigiosin: the "red" and "yellow" ones with absorption maxima at 535 and 460-470 nm, respectively. The absorption spectrum of prodigiosin in the native pigment-protein complex was different from the spectrum of the pigment dissolved in ethanol and resembled that of the cell suspension in the presence of an additional absorption maximum at 500 nm.

摘要

粘质沙雷氏菌的色素沉着取决于培养基的成分。在甘油 - 蛋白胨培养基和含有乙酸盐的培养基上生长的培养物分别为红色和黄色(橙黄色),颜色取决于环境pH值。在甘油 - 蛋白胨培养基上生长(肉眼可见为红色)的粘质沙雷氏菌细胞含有两种灵菌红素形式:“红色”和“黄色”形式,其吸收最大值分别在535和460 - 470 nm处。天然色素 - 蛋白质复合物中灵菌红素的吸收光谱与溶解在乙醇中的色素光谱不同,并且类似于细胞悬液的光谱,在500 nm处有一个额外的吸收最大值。

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