State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250000, China.
Shandong Academy of Agricultural Sciences, Jinan 250000, China.
Molecules. 2021 Apr 9;26(8):2156. doi: 10.3390/molecules26082156.
Prodigiosin is a red pigment produced by with anticancer, antimalarial, and antibacterial effects. In this study, we extracted and identified a red pigment from a culture of strain ZPG19 and investigated its effect on the growth performance and intestinal microbiota of Kunming mice. High-performance liquid chromatography/mass spectrometry revealed that the pigment had a mass-to-charge ratio (/) of 324.2160, and thus it was identified as prodigiosin. To investigate the effect of prodigiosin on the intestinal microbiota, mice (n = 5) were administered 150 μg/kg/d prodigiosin (crude extract, 95% purity) via the drinking water for 18 days. Administration of prodigiosin did not cause toxicity in mice. High-throughput sequencing analysis revealed that prodigiosin altered the cecum microbiota abundance and diversity; the relative abundance of significantly decreased, whereas significantly increased. This finding indicates that oral administration of prodigiosin has a beneficial effect on the intestinal microbiota of mice. As prodigiosin is non-toxic to mouse internal organs and improves the mouse intestinal microbiota, we suggest that it is a promising candidate drug to treat intestinal inflammation.
灵菌红素是一种红色素，由 产生，具有抗癌、抗疟和抗菌作用。在这项研究中，我们从 菌株 ZPG19 的培养物中提取并鉴定了一种红色素，并研究了它对昆明小鼠生长性能和肠道微生物群的影响。高效液相色谱/质谱联用仪显示该色素的质荷比 (/m/z) 为 324.2160，因此将其鉴定为灵菌红素。为了研究灵菌红素对肠道微生物群的影响，将小鼠（n = 5）通过饮用水给予 150 μg/kg/d 灵菌红素（粗提物，纯度 95%），持续 18 天。灵菌红素的给予未引起小鼠毒性。高通量测序分析显示，灵菌红素改变了盲肠微生物群的丰度和多样性； 的相对丰度显著降低，而 的相对丰度显著增加。这一发现表明，口服灵菌红素对小鼠肠道微生物群有有益的影响。由于灵菌红素对小鼠内脏器官无毒，并且改善了小鼠肠道微生物群，因此我们认为它是治疗肠道炎症的有前途的候选药物。
