Conti Bruno J, Santiago Karina B, Búfalo Michelle C, Herrera Yahima F, Alday Efrain, Velazquez Carlos, Hernandez Javier, Sforcin José M
Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, São Paulo, Brazil.
Food and Pharmacy Institute, Havana University, Havana, Cuba.
J Pharm Pharmacol. 2015 Oct;67(10):1431-8. doi: 10.1111/jphp.12431. Epub 2015 Apr 29.
Propolis has been used in folk medicine in different regions of the world including Latin America. Propolis is a resinous mixture of substances collected by honey bees from several botanical sources, and its composition contains a rich chemical variety, depending on the geographical area and plant sources. Our aim was to compare the modulatory effect of propolis samples from three different countries of Latin America (Brazil, Cuba and Mexico) on pro- and anti-inflammatory cytokine production (tumor necrosis factor (TNF)-α and interleukin (IL)-10, respectively) by human monocytes.
Cells were incubated with propolis for 18 h at 37°C. Cell viability was assessed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide method, and cytokine production was determined by ELISA.
All samples did not affect monocyte viability. Brazilian propolis stimulated both TNF-α and IL-10 production by monocytes. Cuban propolis stimulated TNF-α and inhibited IL-10 production, while Mexican sample exerted the opposite effect, inhibiting TNF-α and stimulating IL-10 production. The major compounds found in Brazilian, Cuban and Mexican propolis samples were artepillin C, isoflavonoids and pinocembrin, respectively.
Brazilian, Cuban and Mexican propolis contained different components that may exert pro- and anti-inflammatory activity depending on concentration, what may provide a novel approach to the development of immunomodulatory drugs containing propolis.
蜂胶已在包括拉丁美洲在内的世界不同地区用于民间医学。蜂胶是蜜蜂从多种植物来源采集的物质的树脂状混合物，其成分因地理区域和植物来源而异，化学种类丰富。我们的目的是比较来自拉丁美洲三个不同国家（巴西、古巴和墨西哥）的蜂胶样品对人单核细胞促炎和抗炎细胞因子产生（分别为肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL)-10）的调节作用。
细胞在 37°C 下与蜂胶孵育 18 小时。通过 3-(4,5-二甲基-噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估细胞活力，并通过 ELISA 测定细胞因子产生。
所有样品均不影响单核细胞活力。巴西蜂胶刺激单核细胞产生 TNF-α 和 IL-10。古巴蜂胶刺激 TNF-α 并抑制 IL-10 的产生，而墨西哥样品则产生相反的效果，抑制 TNF-α 并刺激 IL-10 的产生。在巴西、古巴和墨西哥蜂胶样品中发现的主要化合物分别是artepillin C、异黄酮和松属素。
巴西、古巴和墨西哥的蜂胶含有不同的成分，这些成分可能根据浓度发挥促炎和抗炎活性，这可能为开发含蜂胶的免疫调节药物提供一种新方法。
