Li Xiyuan, Feng Shaolong, Hu Yaxi, Sheng Wei, Zhang Yan, Yuan Shifang, Zeng Haishan, Wang Shuo, Lu Xiaonan
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Univ. of Science and Technology, Tianjin, 300457, China.
Food, Nutrition and Health Program, Faculty of Land and Food Systems, Univ. of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.
J Food Sci. 2015 Jun;80(6):C1196-201. doi: 10.1111/1750-3841.12876. Epub 2015 Apr 28.
We integrated immunological separation and surface-enhanced Raman spectroscopy (SERS) to detect melamine in milk. Antimelamine was produced by New Zealand white rabbits following the injection with melamine hapten-ovalbumin immunogen. Melamine was separated from milk by binding to the converted protein G-antimelamine complex. After releasing antimelamine and melamine from the complex, the eluents were deposited directly onto the silver dendrite SERS-active substrate for spectral collection. Multivariate statistical analysis including unsupervised principal component analysis and supervised soft independent modeling of class analogy validated the feasibility of applying this method to detect trace levels of melamine in milk. The limit of detection can be as low as 0.79×10(-3) mmol/L. The overall analysis can be completed in 20 min, thus, it is a high-throughput technique to screen for melamine in milk samples.
我们将免疫分离与表面增强拉曼光谱（SERS）相结合来检测牛奶中的三聚氰胺。用三聚氰胺半抗原-卵清蛋白免疫原注射新西兰白兔后制备抗三聚氰胺。三聚氰胺通过与转化后的蛋白G-抗三聚氰胺复合物结合而从牛奶中分离出来。从复合物中释放出抗三聚氰胺和三聚氰胺后，将洗脱液直接沉积在银树枝状SERS活性基底上进行光谱采集。包括无监督主成分分析和有监督的类软独立建模在内的多变量统计分析验证了应用该方法检测牛奶中痕量三聚氰胺的可行性。检测限可低至0.79×10(-3) mmol/L。整个分析可在20分钟内完成，因此，它是一种用于筛选牛奶样品中三聚氰胺的高通量技术。
