Department of Pharmacy, Shanghai Chang Hai Hospital, Second Military Medical University, China; Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, China.
Food Chem. 2017 Jun 1;224:382-389. doi: 10.1016/j.foodchem.2016.12.040. Epub 2016 Dec 18.
Methods for the on-site analysis of food contaminants are in high demand. Although portable Raman spectroscopy is commonly used to test food on-site, it can be challenge to achieve this goal with rapid detection and inexpensive substrate. In this study, we detected trace food contaminants in samples of whole milk powder using the methods that combined chromatography with surface-enhanced Raman scattering detection (SERS). We developed a simple and efficient technique to fabricate the paper with chitosan-modified silver nanoparticles as a SERS-active substrate. The soaking time of paper and the concentration of chitosan solution were optimized for chromatographic separation and SERS detection. We then studied the separation properties for real applications including complex sample matrices, and detected melamine at 1mg/L, dicyandiamide at 100mg/L and sodium sulfocyanate at 10mg/L in whole milk powder. As such, our methods have great potential for field-based detection of milk contaminants.
现场分析食品污染物的方法需求很大。虽然便携式拉曼光谱仪常用于现场测试食品，但要实现快速检测和低成本基底的目标仍具有挑战性。在本研究中，我们结合色谱法和表面增强拉曼散射检测（SERS）的方法，检测了全脂奶粉样品中的痕量食品污染物。我们开发了一种简单高效的技术，用壳聚糖修饰的银纳米粒子制备了具有 SERS 活性的纸基。优化了纸的浸泡时间和壳聚糖溶液的浓度，以实现色谱分离和 SERS 检测。然后，我们研究了实际应用中的分离特性，包括复杂的样品基质，并在全脂奶粉中检测到了 1mg/L 的三聚氰胺、100mg/L 的双氰胺和 10mg/L 的硫氰酸钠。因此，我们的方法在现场检测牛奶污染物方面具有很大的潜力。
