Brzuzan P, Woźny M, Wolińska-Nizioł L, Piasecka A, Florczyk M, Jakimiuk E, Góra M, Łuczyński M K, Gajecki M
Pol J Vet Sci. 2015;18(1):29-38. doi: 10.1515/pjvs-2015-0004.
To improve our knowledge of the role of microRNAs (miRs) in responses of the porcine digestive system to two Fusarium mycotoxins, zearalenone (ZEN) and deoxynivalenol (DON), we examined the expression of 7 miRs (miR-9, miR-15a, miR-21, miR-34a, miR-122, miR-125b, and miR-192), previously found to be deregulated in diseased liver and colon cells. In this study, immature gilts were exposed to NOEL doses of ZEN (40 μg/kg/d), DON (12 μg/kg/d), ZEN + DON (40 + 12 μg/kg/d), andplacebo (negative control group) for 7, 14, 21, 28, 35, and 42 days. Before the treatment, expression levels of the selected miRs were measured in the liver, the duodenum, the jejunum, and the ascending and the descending colon of the gilts. Hierarchical clustering of the tissues by their miR expression profiles was consistent with what would be expected based on the anatomical locations and the physiological functions of the organs, suggesting that functions of the miRs are related to the specificities of the tissues in which they are expressed. A subset of 2 pairs of miRs (miR-21+miR-192 and miR-15a+miR-34a), which were assigned to two distinct clusters based on their tissue abundance, was then evaluated in the liver and the ascending and the descending colon during the treatment. The most meaningful results were obtained from the ascending colon, where a significant effect of the treatment was observed, suggesting that during the exposure to mycotoxins, the pathways involved in cell proliferation and survival were disordered. Changes in miR expression in the liver and the descending colon of the treated gilts were smaller, and were associated more with treatment duration than the exposure to ZEN, DON, or ZEN + DON. Further research should focus on identification of genes whose expression is regulated by these aberrantly expressed miRs. This should facili- tate understanding of the miRNA-regulated biological effects of mycotoxins.
为了增进我们对微小RNA（miR）在猪消化系统对两种镰刀菌霉菌毒素——玉米赤霉烯酮（ZEN）和脱氧雪腐镰刀菌烯醇（DON）反应中所起作用的了解，我们检测了7种miR（miR - 9、miR - 15a、miR - 21、miR - 34a、miR - 122、miR - 125b和miR - 192）的表达，此前发现这些miR在患病的肝脏和结肠细胞中表达失调。在本研究中，将未成年后备母猪暴露于无明显有害作用剂量的ZEN（40μg/kg/d）、DON（12μg/kg/d）、ZEN + DON（40 + 12μg/kg/d）以及安慰剂（阴性对照组）中，持续7、14、21、28、35和42天。在处理前，测定后备母猪肝脏、十二指肠、空肠以及升结肠和降结肠中所选miR的表达水平。根据组织的miR表达谱进行层次聚类，结果与基于器官的解剖位置和生理功能所预期的一致，这表明miR的功能与其所表达组织的特异性相关。然后，根据其在组织中的丰度分为两个不同簇的2对miR（miR - 21 + miR - 192和miR - 15a + miR - 34a），在处理过程中对其在肝脏以及升结肠和降结肠中的情况进行评估。最有意义的结果来自升结肠，在那里观察到了处理的显著效果，并表明在接触霉菌毒素期间，细胞增殖和存活所涉及的途径紊乱。处理后的后备母猪肝脏和降结肠中miR表达的变化较小，且更多地与处理持续时间相关，而非与接触ZEN、DON或ZEN + DON相关。进一步的研究应聚焦于鉴定那些表达受这些异常表达的miR调控的基因。这将有助于理解霉菌毒素的miRNA调控生物学效应。
