Kong Kalyan, Islam Md Sofiqul, Nassar Mohannad, Hiraishi Noriko, Otsuki Masayuki, Yiu Cynthia K Y, Tagami Junji
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong.
J Mech Behav Biomed Mater. 2015 Aug;48:145-152. doi: 10.1016/j.jmbbm.2015.03.027. Epub 2015 Apr 6.
This study examined the effect of phytic acid (IP6) in stabilizing the morphology of dentine collagen network and resin-dentine bonding.
Dentine beams were fully demineralized with 10% phosphoric acid (PA) or 1% IP6 (pH 1.2). PA-demineralized beams were divided into three groups: (a) no further treatment (control), (b) treatment with 5% glutaraldehyde (GA) for 1 h and (c) treatment with 1% IP6 (pH 7) for 1 h. IP6-demineralized beams received no further treatment. The beams were then subjected to ultimate tensile strength (UTS) testing. Dentine micromorphology evaluation was performed using a field-emission scanning electron microscope (FE-SEM). Dentine disks were etched with 35% PA for 15 s or 1% IP6 for 30s. PA-etched dentine disks were divided into three groups as (a), (b) and (c) as for UTS testing, but the treatment with GA or IP6 was done in 1min. For microtensile bond strength (µTBS) testing, flat dentine surfaces etched with PA or IP6 were blot-dried (wet dentine) or air-dried for 10s (dry dentine) and bonded with an etch-and-rinse adhesive followed by composite build-up.
IP6-demineralized dentine showed significantly higher UTS, when compared to PA-demineralized dentine. GA and IP6 significantly improved UTS of PA-demineralized dentine. FE-SEM observation revealed that dentine collagen network was preserved by GA and IP6. No significant difference in µTBS was found between the wet and dry IP6-etched dentine groups.
IP6 etching showed a structural stabilizing effect on demineralized dentine matrix and produced good resin-dentine bonding, regardless of dentine moistness or dryness.
本研究考察了植酸（IP6）对牙本质胶原网络形态稳定性及树脂 - 牙本质粘结的影响。
用10%磷酸（PA）或1% IP6（pH 1.2）将牙本质梁完全脱矿。PA脱矿的牙本质梁分为三组：（a）不做进一步处理（对照组），（b）用5%戊二醛（GA）处理1小时，（c）用1% IP6（pH 7）处理1小时。IP6脱矿的牙本质梁不做进一步处理。然后对这些牙本质梁进行极限拉伸强度（UTS）测试。使用场发射扫描电子显微镜（FE - SEM）进行牙本质微观形态评估。用35% PA蚀刻牙本质盘15秒或用1% IP6蚀刻30秒。PA蚀刻的牙本质盘如UTS测试那样分为（a）、（b）和（c）三组，但用GA或IP6处理1分钟。对于微拉伸粘结强度（µTBS）测试，用PA或IP6蚀刻的平坦牙本质表面用吸水纸吸干（湿牙本质）或风干10秒（干牙本质），然后用一种酸蚀冲洗粘结剂粘结，随后进行复合树脂堆积。
与PA脱矿的牙本质相比，IP6脱矿的牙本质显示出显著更高的UTS。GA和IP6显著提高了PA脱矿牙本质的UTS。FE - SEM观察显示，GA和IP6使牙本质胶原网络得以保留。在湿和干的IP6蚀刻牙本质组之间，µTBS未发现显著差异。
无论牙本质湿润还是干燥，IP蚀刻对脱矿牙本质基质均显示出结构稳定作用，并产生良好的树脂 - 牙本质粘结。
