Nassar Mohannad, Hiraishi Noriko, Islam Md Sofiqul, Romero Maria Jrh, Otsuki Masayuki, Tagami Junji
Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah, UAE.
Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Restor Dent Endod. 2020 Aug 24;45(4):e44. doi: 10.5395/rde.2020.45.e44. eCollection 2020 Nov.
Phytic acid (IP6), a naturally occurring agent, has been previously reported as a potential alternative to ethylenediaminetetraacetic acid (EDTA). However, its effect on adhesion to sodium hypochlorite (NaOCl)-treated dentin and its interactions with NaOCl have not been previously reported. Thus, in this study, the effects of IP6 on resin adhesion to NaOCl-treated dentin and the failure mode were investigated and the interactions between the used agents were analyzed.
Micro-tensile bond strength (µTBS) testing was performed until failure on dentin treated with either distilled water (control), 5% NaOCl, or 5% NaOCl followed with chelators: 17% EDTA for 1 minute or 1% IP6 for 30 seconds or 1 minute. The failed specimens were assessed under a scanning electron microscope. The reaction of NaOCl with EDTA or IP6 was analyzed in terms of temperature, pH, effervescence, and chlorine odor, and the effects of the resulting mixtures on the color of a stained paper were recorded.
The µTBS values of the control and NaOCl with chelator groups were not significantly different, but were all significantly higher than that of the group treated with NaOCl only. In the failure analysis, a distinctive feature was the presence of resin tags in samples conditioned with IP6 after treatment with NaOCl. The reaction of 1% IP6 with 5% NaOCl was less aggressive than the reaction of the latter with 17% EDTA.
IP6 reversed the adverse effects of NaOCl on resin-dentin adhesion without the chlorine-depleting effect of EDTA.
植酸（IP6）是一种天然存在的物质，此前有报道称它可能是乙二胺四乙酸（EDTA）的替代品。然而，其对次氯酸钠（NaOCl）处理过的牙本质的黏附作用以及与NaOCl的相互作用此前尚未见报道。因此，在本研究中，研究了IP6对树脂与NaOCl处理过的牙本质黏附的影响及失效模式，并分析了所用试剂之间的相互作用。
进行微拉伸粘结强度（µTBS）测试，直至用蒸馏水（对照）、5% NaOCl或5% NaOCl加螯合剂处理的牙本质发生失效：17% EDTA处理1分钟或1% IP6处理30秒或1分钟。在扫描电子显微镜下评估失效的标本。从温度、pH值、气泡产生和氯气味方面分析NaOCl与EDTA或IP6的反应，并记录所得混合物对染色纸颜色的影响。
对照组和NaOCl加螯合剂组的µTBS值无显著差异，但均显著高于仅用NaOCl处理的组。在失效分析中，一个显著特征是在用NaOCl处理后用IP6处理的样本中存在树脂突。1% IP6与5% NaOCl的反应比后者与17% EDTA的反应温和。
IP6可逆转NaOCl对树脂-牙本质黏附的不利影响，且没有EDTA的耗氯作用。
