Hismiogullari Adnan A, Hismiogullari Sahver E, Karaca Omur, Sunay Fatma B, Paksoy Serpil, Can Mehmet, Kus Iter, Seyrek Kamil, Yavuz Ozlem
Department of Medical Biochemistry, School of Medicine, Balikesir University, Balikesir, Turkey.
Department of Pharmacology and Toxicology, School of Veterinary Medicine, Balkesir University, Balikesir, Turkey.
Pharmacol Rep. 2015 Jun;67(3):410-6. doi: 10.1016/j.pharep.2014.10.021. Epub 2014 Nov 11.
The aim of the present study was to examine the protective effect of curcumin (CUR) on carbon tetrachloride (CCl4)-induced nephrotoxicity to evaluate the detailed mechanisms by which CUR exerts its protective action.
Thirty male Wistar-Albino rats weighing 250-300 g were randomly divided into three groups: administrations of olive oil (control, po), CCl4 (0.5mg/kg in olive oil sc) every other day for 3 weeks, and CCl4 (0.5mg/kg in olive oil sc) plus CUR (200mg/kg) every day for 3 weeks.
Administration of CCl4 significantly (p<0.001) increased the levels of renal function test such as creatinine and blood urea nitrogen (BUN). Furthermore, treatment of CCl4 significantly elevated the oxidant status of renal tissues while decreasing its anti-oxidant status (p<0.001). CUR displayed a renal protective effect as evident by significant decrease in inflammation and apoptosis during histopathological examination. The administration of CCl4 resulted in an increase in malondialdehyde (MDA) production due to an increase in membrane lipid peroxidation; however, the administration of CUR attenuated this, probably via its antioxidant and free radical scavenging properties.
The finding of our study indicates that CUR may have an important role to play in protecting the kidney from oxidative insult.
本研究旨在探讨姜黄素（CUR）对四氯化碳（CCl4）诱导的肾毒性的保护作用，以评估CUR发挥其保护作用的详细机制。
将30只体重250 - 300克的雄性Wistar - Albino大鼠随机分为三组：给予橄榄油（对照组，经口）；每隔一天给予CCl4（0.5毫克/千克，溶于橄榄油，皮下注射），共3周；每天给予CCl4（0.5毫克/千克，溶于橄榄油，皮下注射）加CUR（200毫克/千克），共3周。
给予CCl4显著（p<0.001）增加了肾功能测试指标如肌酐和血尿素氮（BUN）的水平。此外，CCl4处理显著提高了肾组织的氧化状态，同时降低了其抗氧化状态（p<0.001）。在组织病理学检查中，CUR显示出肾脏保护作用，表现为炎症和凋亡显著减少。给予CCl4由于膜脂质过氧化增加导致丙二醛（MDA）生成增加；然而，给予CUR可能通过其抗氧化和自由基清除特性减轻了这种情况。
我们的研究结果表明，CUR可能在保护肾脏免受氧化损伤方面发挥重要作用。
