Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Department of Radiology, The Second Hospital of Jilin University, Changchun 130041, China.
Biomaterials. 2015 Jul;56:206-18. doi: 10.1016/j.biomaterials.2015.04.005. Epub 2015 Apr 18.
Since understanding the healthy status of gastrointestinal tract (GI tract) is of vital importance, clinical implementation for GI tract-related disease have attracted much more attention along with the rapid development of modern medicine. Here, a multifunctional theranostic system combining X-rays/CT/photothermal/photoacoustic mapping of GI tract and imaging-guided photothermal anti-bacterial treatment is designed and constructed. PEGylated W18O49 nanosheets (PEG-W18O49) are created via a facile solvothermal method and an in situ probe-sonication approach. In terms of excellent colloidal stability, low cytotoxicity, and neglectable hemolysis of PEG-W18O49, we demonstrate the first example of high-performance four-modal imaging of GI tract by using these nanosheets as contrast agents. More importantly, due to their intrinsic absorption of NIR light, glutaraldehyde-modified PEG-W18O49 are successfully applied as fault-free targeted photothermal agents for imaging-guided killing of bacteria on a mouse infection model. Critical to pre-clinical and clinical prospects, long-term toxicity is further investigated after oral administration of these theranostic agents. These kinds of tungsten-based nanomaterials exhibit great potential as multi-modal contrast agents for directed visualization of GI tract and anti-bacterial agents for phothothermal sterilization.
由于了解胃肠道（GI 道）的健康状况至关重要，随着现代医学的快速发展，与胃肠道相关疾病的临床治疗方法受到了更多关注。在这里，设计并构建了一种多功能治疗系统，该系统结合了胃肠道的 X 射线/CT/光热/光声成像和成像引导的光热抗菌治疗。通过简便的溶剂热法和原位探针超声法制备了聚乙二醇化 W18O49 纳米片（PEG-W18O49）。由于 PEG-W18O49 具有出色的胶体稳定性、低细胞毒性和可忽略不计的溶血作用，我们首次将这些纳米片作为造影剂用于胃肠道的高性能四模态成像。更重要的是，由于其对近红外光的固有吸收，戊二醛修饰的 PEG-W18O49 成功地用作无缺陷的靶向光热剂，用于在小鼠感染模型上进行成像引导的杀菌。对于临床前和临床前景至关重要的是，在口服这些治疗剂后进一步研究了长期毒性。这些基于钨的纳米材料作为胃肠道定向可视化的多模态造影剂和光热杀菌的抗菌剂具有很大的潜力。
