Ge Shuping, Wang Yi, Tian Jie, Lei Daoxi, Yu Qingsong, Wang Guixue
College of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China.
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, China.
J Biomed Mater Res B Appl Biomater. 2016 Apr;104(3):482-7. doi: 10.1002/jbm.b.33388. Epub 2015 May 1.
Magnesium alloys are being actively studied for intravascular stent applications because of their good mechanical strength and biocompatibility. To rule out the high allergenicity of nickel and neurotoxicity of aluminum element, four kinds of WE magnesium alloys (where "W" represents the metallic element Y and "E" represents mixed rare earth (RE) elements; Y: 2.5, 5.0, 6.5, and 7.5 wt %; Nd: 1.0, 2.6, 2.5, and 4.2 wt %; Zr: 0.8 wt %) were chosen for in vitro investigation of their biocompatibility using cell culture. The results showed that, with the increase of rare earth elements in WE magnesium alloys, fibrinogen adsorption decreased and coagulation function was improved. It was also found that WE magnesium alloys promoted the adhesion of endothelial cells. With the increase of adhesion time, adhered cell numbers increased gradually. With 25% extracts, all the WE alloys promoted cell migration, while 100% extracts were not conducive to cell migration. Based on the above results, WE magnesium alloys 5.0WE (5.0Y-2.6Nd-0.8Zr) and 6.5WE (6.5Y-2.5Nd-0.8Zr) have better biocompatibility as compared with that with 2.5WE (2.5Y-1.0Nd-0.8Zr) and 7.5WE (7.5Y-4.2Nd-0.8Zr), and could be as the promising candidate materials for medical stent applications.
由于镁合金具有良好的机械强度和生物相容性，因此正在积极研究其在血管内支架中的应用。为了排除镍的高致敏性和铝元素的神经毒性，选择了四种WE镁合金（其中“W”代表金属元素钇，“E”代表混合稀土（RE）元素；钇：2.5、5.0、6.5和7.5 wt%；钕：1.0、2.6、2.5和4.2 wt%；锆：0.8 wt%），使用细胞培养对其生物相容性进行体外研究。结果表明，随着WE镁合金中稀土元素含量的增加，纤维蛋白原吸附减少，凝血功能得到改善。还发现WE镁合金促进内皮细胞的黏附。随着黏附时间的增加，黏附细胞数量逐渐增加。在25%提取物的情况下，所有WE合金都促进细胞迁移，而100%提取物则不利于细胞迁移。基于上述结果，与含2.5WE（2.5Y-1.0Nd-0.8Zr）和7.5WE（7.5Y-4.2Nd-0.8Zr）的合金相比，5.0WE（5.0Y-2.6Nd-0.8Zr）和6.5WE（6.
