Hillwig Melissa S, Chiozza Mariana, Casteel Clare L, Lau Siau Ting, Hohenstein Jessica, Hernández Enrique, Jander Georg, MacIntosh Gustavo C
Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
Boyce Thompson Institute for Plant Research, Ithaca, NY, 14853, USA.
Mol Plant Pathol. 2016 Feb;17(2):225-35. doi: 10.1111/mpp.12274. Epub 2015 Jun 7.
Comparison of Arabidopsis thaliana (Arabidopsis) gene expression induced by Myzus persicae (green peach aphid) feeding, aphid saliva infiltration and abscisic acid (ABA) treatment showed a significant positive correlation. In particular, ABA-regulated genes are over-represented among genes that are induced by M. persicae saliva infiltration into Arabidopsis leaves. This suggests that the induction of ABA-related gene expression could be an important component of the Arabidopsis-aphid interaction. Consistent with this hypothesis, M. persicae populations induced ABA production in wild-type plants. Furthermore, aphid populations were smaller on Arabidopsis aba1-1 mutants, which cannot synthesize ABA, and showed a significant preference for wild-type plants compared with the mutant. Total free amino acids, which play an important role in aphid nutrition, were not altered in the aba1-1 mutant line, but the levels of isoleucine (Ile) and tryptophan (Trp) were differentially affected by aphids in wild-type and mutant plants. Recently, indole glucosinolates have been shown to promote aphid resistance in Arabidopsis. In this study, 4-methoxyindol-3-ylmethylglucosinolate was more abundant in the aba1-1 mutant than in wild-type Arabidopsis, suggesting that the induction of ABA signals that decrease the accumulation of defence compounds may be beneficial for aphids.
对由桃蚜（绿桃蚜）取食、蚜虫唾液浸润以及脱落酸（ABA）处理诱导的拟南芥基因表达进行比较，结果显示存在显著正相关。特别是，在由桃蚜唾液浸润拟南芥叶片所诱导的基因中，ABA调控的基因占比过高。这表明ABA相关基因表达的诱导可能是拟南芥与蚜虫相互作用的一个重要组成部分。与该假设一致，桃蚜种群在野生型植物中诱导了ABA的产生。此外，在无法合成ABA的拟南芥aba1 - 1突变体上蚜虫种群数量较少，并且与突变体相比，蚜虫对野生型植物表现出显著偏好。在蚜虫营养中起重要作用的总游离氨基酸在aba1 - 1突变体系中未发生改变，但异亮氨酸（Ile）和色氨酸（Trp）的水平在野生型和突变型植物中受到蚜虫的不同影响。最近，吲哚硫代葡萄糖苷已被证明可促进拟南芥对蚜虫的抗性。在本研究中，4 - 甲氧基吲哚 - 3 - 基甲基硫代葡萄糖苷在aba1 - 1突变体中比在野生型拟南芥中更为丰富，这表明降低防御化合物积累的ABA信号诱导可能对蚜虫有益。
