Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
Iran J Basic Med Sci. 2015 Mar;18(3):221-7.
Oxytetracycline (OTC) is a broad spectrum antibiotic widely used for treatment of a wide range of infections. However, its improper human and animal use leads to toxic effects, including hepatonephrotoxicity. Our objective was to evaluate protective effects of Nigella sativa oil (NSO) and/or ascorbic acid (AA), against OTC-induced hepatonephrotoxicity in rabbits.
Forty male white New Zealand rabbits were divided into 5 groups of eight each. The 1(st) group (control) was given saline. The 2(nd) group was given OTC (200 mg/kg, orally). The 3(rd) and 4(th) groups were orally administered NSO and AA (2 ml/kg and 200 mg/kg respectively) 1 hr before OTC administration at the same dose regimen used for the 2(nd) group. Both NSO and AA were given in combination for the 5(th) group along with OTC administration. Serum biochemical parameters related to liver and kidney injury were evaluated, and lipid peroxidation as well as antioxidant markers in hepatic and renal tissues were examined.
OTC-treated animals revealed significant alterations in serum biochemical hepato-renal injury markers, and showed a markedly increase in hepato-renal lipid peroxidation and inhibition in tissue antioxidant biomarkers. NSO and AA protect against OTC-induced serum and tissue biochemical alterations when each of them is used alone or in combination along with OTC treatment. Furthermore, both NSO and AA produced synergetic hepatoprotective and antioxidant properties.
The present study revealed the preventive role of NSO and/or AA against the toxic effects of OTC through their free radical-scavenging and potent antioxidant activities.
土霉素（OTC）是一种广谱抗生素，广泛用于治疗多种感染。然而，其在人和动物中的不当使用会导致毒性作用，包括肝肾病毒性。我们的目的是评估黑种草籽油（NSO）和/或抗坏血酸（AA）对兔 OTC 诱导的肝肾病毒性的保护作用。
40 只雄性新西兰白兔分为 5 组，每组 8 只。第 1 组（对照组）给予生理盐水。第 2 组给予 OTC（200mg/kg，口服）。第 3 组和第 4 组分别在给予 OTC（第 2 组相同剂量方案）前 1 小时口服 NSO 和 AA（2ml/kg 和 200mg/kg）。第 5 组在给予 OTC 的同时，联合给予 NSO 和 AA。评估与肝肾功能损伤相关的血清生化参数，并检测肝肾功能组织中的脂质过氧化和抗氧化标志物。
接受 OTC 治疗的动物显示血清生化肝肾功能损伤标志物显著改变，并显示肝肾功能脂质过氧化明显增加，组织抗氧化生物标志物抑制。当 NSO 和 AA 单独使用或与 OTC 联合使用时，均可预防 OTC 诱导的血清和组织生化改变。此外，NSO 和 AA 均具有协同的肝保护和抗氧化作用。
本研究表明，NSO 和/或 AA 通过清除自由基和发挥强大的抗氧化活性，对 OTC 的毒性作用具有预防作用。
