Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (SCIFAR), University of Messina, Viale Annunziata, 98168 Messina, Italy.
J Anal Methods Chem. 2015;2015:951748. doi: 10.1155/2015/951748. Epub 2015 Apr 7.
Mushrooms are sources of food, medicines, and agricultural means. Not much is reported in the literature about wild species of the Mediterranean flora, although many of them are traditionally collected for human consumption. The knowledge of their chemical constituents could represent a valid tool for both taxonomic and physiological characterizations. In this work, a headspace-solid-phase microextraction (HS-SPME) method coupled with GC-MS and GC-FID was developed to evaluate the volatile profiles of ten wild mushroom species collected in South Italy. In addition, in order to evaluate the potential of this analytical methodology for true quantitation of volatiles, samples of the cultivated species Agaricus bisporus were analyzed. The choice of this mushroom was dictated by its ease of availability in the food market, due to the consistent amounts required for SPME method development. For calibration of the main volatile compounds, the standard addition method was chosen. Finally, the assessed volatile composition of A. bisporus was monitored in order to evaluate compositional changes occurring during storage, which represents a relevant issue for such a wide consumption edible product.
蘑菇既是食物，也是药品和农业生产资料的来源。有关地中海植物区系野生物种的文献报道并不多，但其中许多物种传统上都被人类采集食用。对其化学成分的了解可能是进行分类和生理特征描述的有效工具。在这项工作中，开发了一种顶空固相微萃取（HS-SPME）方法，结合 GC-MS 和 GC-FID，用于评估在意大利南部采集的十种野生蘑菇物种的挥发性成分谱。此外，为了评估这种分析方法对挥发性物质进行真正定量的潜力，还对栽培品种双孢蘑菇的样本进行了分析。选择这种蘑菇是因为它在食品市场上很容易获得，这是由于 SPME 方法开发所需的稳定数量。对于主要挥发性化合物的校准，选择了标准添加法。最后，监测了双孢蘑菇的评估挥发性成分，以评估在储存过程中发生的成分变化，这对于如此广泛食用的产品来说是一个相关的问题。
