Abidi Anouar, Aissani Nadhem, Sebai Hichem, Serairi Raja, Kourda Nadia, Ben Khamsa Saloua
a Unit Research 03/UR/08-05, Pulmonary Fibrosis: Prevention & Treatment, Faculty of Medicine of Tunis , Tunis , Tunisia.
b Laboratory of Functional Physiology and Valorization of Bioresources , High Institute of Biotechnology of Beja, University of Jendouba , Beja , Tunisia.
Nutr Cancer. 2017 Apr;69(3):490-497. doi: 10.1080/01635581.2017.1283423. Epub 2017 Feb 17.
We aimed in the present study to investigate the protective effect of Pistacia lentiscus oil against bleomycin-induced lung fibrosis as well as the involvement of oxidative stress in such protection. In this respect, adult male Wistar rats were used and divided into three groups of twenty each: control (NaCl, 0.9%), bleomycin, and bleomycin (4 mg/kg b.w.) + P. lentiscus oil (3 g/kg, b.w.). Animals were pretreated for 30 days before the induction of fibrosis by bleomycin and 1 wk after the induction of fibrosis. The oil principal compounds detected by gas chromatography analysis are: Linoleic and palmitic acids (70.6 and 24.7%, respectively). Our data demonstrated that P. lentiscus oil protected against bleomycin-induced fibrosis as evidenced by TGFβ immunostaining increase in lungs fibrocytes as well as inflammatory infiltrate. We also showed that acute bleomycin-induced fibrosis was accompanied by an oxidative stress in lung tissue as assessed by an increase of lipid peroxidation as well as antioxidant enzyme activities depletion such as superoxide dismutase (SOD) and catalase (CAT). More importantly, P. lentiscus oil treatment reversed all bleomycin-induced oxidative stress parameters disturbances. In conclusion, we suggest that P. lentiscus oil had potent protective effects against bleomycin-induced fibrosis due in part to its antioxidant properties.
在本研究中，我们旨在探究乳香黄连木油对博来霉素诱导的肺纤维化的保护作用，以及氧化应激在此类保护中的作用。在这方面，使用成年雄性Wistar大鼠，并将其分为三组，每组20只：对照组（0.9%氯化钠）、博来霉素组，以及博来霉素（4毫克/千克体重）+乳香黄连木油（3克/千克体重）组。在博来霉素诱导纤维化前30天及诱导纤维化后1周对动物进行预处理。通过气相色谱分析检测到的油的主要成分是：亚油酸和棕榈酸（分别为70.6%和24.7%）。我们的数据表明，乳香黄连木油可预防博来霉素诱导的纤维化，肺成纤维细胞中转化生长因子β免疫染色增加以及炎症浸润证明了这一点。我们还表明，通过脂质过氧化增加以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等抗氧化酶活性降低评估，急性博来霉素诱导的纤维化伴有肺组织中的氧化应激。更重要的是，乳香黄连木油处理逆转了所有博来霉素诱导的氧化应激参数紊乱。总之，我们认为乳香黄连木油对博来霉素诱导的纤维化具有强大的保护作用，部分原因在于其抗氧化特性。
