Hemmati Ali Asghar, Foroozan Maryam, Houshmand Gholamreza, Moosavi Zahra Beigom, Bahadoram Mohammad, Maram Nader Shakiba
Medical Student, Ahvaz Jundishapur University of Medical Sciences, Ahvaz- Iran.
Glob J Health Sci. 2014 Oct 29;7(3):52-8. doi: 10.5539/gjhs.v7n3p52.
Reducing the wound healing time is crucial in wound as it lowers the chance of infection and decreases complications and cost. Grape seed extract has the ability to release endothelial growth factor and its topical application results in contraction and closure of the skin wound. Furthermore, it possesses antioxidant and antibacterial properties. In several studies it has been proved effective in animals. Therefore, due to low side effects and recognition of herbal medicine, we decided to evaluate the effect of grape seed extract 2% herbal cream on human skin lesions.
This study is a double blind clinical trial conducted on two groups of treatment and placebo. Surgery was performed on skin lesions such as skin tags and moles which were found on the neck, trunk and limbs (except for face). After enrollment and obtaining informed consent from participants, they were randomized into two groups of treatment and placebo. Excision of the lesions was done by surgical scissors. The lesions got restored by secondary intention method. After the first day of treatment, the patients were visited on the 3rd, 7th, 10th, 14th, and 21st day. Grape seed extract cream 2% was produced and coded by the Faculty of Pharmacy, Ahvaz University of Medical Sciences. In order to compare the two groups, T-test was used. For time assessing, analysis of variance with repeated measures was employed.
The results showed complete repair of wounds averagely on day 8 for the treatment group and on day 14 for the placebo group, which was clearly significant in terms of statistical difference (p=0.00).
Proanthocyanidins in grape seed extract trigger the release of vascular endothelial growth factor and its topical application causes wound contraction and closure. Curing skin lesions with grape seed extract caused proliferation areas with protected boundaries in epithelium, increased cell density and increased deposition of connective tissue at the wound site which in general improves cellular structure in wound. In addition, its anti-inflammatory and anti-microbial properties are effective in wound healing.
缩短伤口愈合时间对于伤口愈合至关重要，因为这会降低感染几率，减少并发症并降低成本。葡萄籽提取物能够释放内皮生长因子，其局部应用可导致皮肤伤口收缩和闭合。此外，它还具有抗氧化和抗菌特性。在多项研究中已证明其对动物有效。因此，鉴于副作用小以及对草药的认可，我们决定评估2%葡萄籽提取物草药乳膏对人体皮肤损伤的效果。
本研究是一项双盲临床试验，对治疗组和安慰剂组进行。对颈部、躯干和四肢（面部除外）出现的皮肤赘生物和痣等皮肤损伤进行手术。在招募参与者并获得知情同意后，将他们随机分为治疗组和安慰剂组。用手术剪刀切除损伤部位。损伤通过二期愈合方法修复。治疗第一天后，在第3、7、10、14和21天对患者进行随访。2%葡萄籽提取物乳膏由阿瓦兹医科大学药学院生产并编码。为比较两组，使用了t检验。对于时间评估，采用重复测量方差分析。
结果显示，治疗组伤口平均在第8天完全修复，安慰剂组在第14天完全修复，在统计学差异方面具有明显显著性（p = 0.00）。
葡萄籽提取物中的原花青素可触发血管内皮生长因子的释放，其局部应用可导致伤口收缩和闭合。用葡萄籽提取物治疗皮肤损伤可使上皮细胞增殖区域边界得到保护，细胞密度增加，伤口部位结缔组织沉积增加，总体上改善了伤口的细胞结构。此外，其抗炎和抗菌特性对伤口愈合有效。
