International and Inter-University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kerala 686560, India; Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110056. doi: 10.1016/j.msec.2019.110056. Epub 2019 Aug 5.
Proanthocyanidins (PCs), a component of grape seed extract (GSE), have recently being used for the treatment of wounds. However, poor absorption, poor stability and rapid elimination from the systemic circulation limit its acceptance. In addressing these problems, we herein report the development of PCs based nanoformulations (PCs/SOLU) for the first time based on 1% GSE and assessed its wound healing potential in-vivo on the wistar rats. GSE and PCs/SOLU nanodispersions 1% were prepared by incorporating them into the ointment base via uniform mixing to form ointment which could be easily applied topically to wounds. The antibacterial activity of PCs/SOLU against gram positive and gram-negative bacteria strains proved that the cell membranes became more permeable with disrupted cell structure. While carrageenan and histamine induced rat paw edema analyses show there was no inflammatory signs in animals treated with 1 wt% of PCs/SOLU nanodispersion. Excision wound measuring about 3 cm in depth was created on the wistar rats. The ointment was applied topically on the wounded site and the wound contraction was measured daily. Grape seed extract (GSE) ointment, ointment base and povidone‑iodine (Povi-Iod) ointment of about 1% was used as the control, positive and negative standards. PCs/SOLU nanodispersion heals the wound by mobilising the fibroblasts in the wound site and inhibits the inflammatory response through decreased expression of monocyte. The macroscopical, immunological and histopathological assessments revealed that PCs/SOLU nanodispersion ointment usage improves the cell adhesion and proliferation.
原花青素（PCs）是葡萄籽提取物（GSE）的一种成分，最近已被用于治疗伤口。然而，其较差的吸收能力、较差的稳定性以及在体循环中迅速消除限制了其应用。为了解决这些问题，我们首次报道了基于 1%GSE 的 PCs 纳米制剂（PCs/SOLU）的开发，并在 Wistar 大鼠体内评估了其潜在的伤口愈合能力。GSE 和 PCs/SOLU 纳米分散体 1%通过均匀混合掺入软膏基质中制成软膏，可方便地局部应用于伤口。PCs/SOLU 对革兰氏阳性和革兰氏阴性细菌的抗菌活性证明，细胞膜的通透性增加，细胞结构被破坏。而角叉菜胶和组胺诱导的大鼠爪肿胀分析表明，用 1%wt PCs/SOLU 纳米分散体治疗的动物没有炎症迹象。在 Wistar 大鼠身上制造约 3cm 深的切除伤口。将软膏局部涂抹在受伤部位，并每天测量伤口收缩情况。将 1%的 GSE 软膏、软膏基质和聚维酮碘（Povi-Iod）软膏用作对照、阳性和阴性标准。PCs/SOLU 纳米分散体通过动员伤口部位的成纤维细胞来愈合伤口，并通过降低单核细胞的表达来抑制炎症反应。宏观、免疫和组织病理学评估表明，PCs/SOLU 纳米分散体软膏的使用可改善细胞黏附和增殖。
