Lee Jang-Wook, Lee Jeong-Ho, Noh Jae Koo, Kim Hyun Chul, Park Choul-Ji, Park Jong-Won, Kim Kyung-Kil
Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Korea.
Dev Reprod. 2014 Dec;18(4):267-74. doi: 10.12717/devrep.2014.18.4.267.
The immune system in teleost fish is not completely developed during embryonic and larval stages, therefore effective innate mechanisms is very important for survival in such an environment. However, the knowledge of the development of immune system assumed to be restricted. In many species, lysozymes have been considered as important genes of the first line immune defense. The early detection of lysozyme mRNA in previous reports, led to the investigation of its presence in oocytes. As a result, c-type lysozyme mRNA transcripts were detected in unfertilized oocytes indicating maternal transfer. Therefore, we investigated the expression patterns of lysozymes in flounder, including the matured oocyte. In our results, c-type lysozyme mRNA was first detected in unfertilized oocyte stage, observed the significantly decreased until hatching stage, and was significantly increased after hatching stage. On the other hand, g-type lysozyme mRNA transcripts were first detected at late neurula stage, and the mRNA level was significantly increased after 20 dph. It may be suggest that maternally supplied mRNAs are selectively degraded prior to the activation of embryonic transcription. This study will be help in understanding the maturation and onset of humoral immunity during development of olive flounder immune system.
硬骨鱼在胚胎和幼体阶段免疫系统尚未完全发育，因此有效的先天免疫机制对于在这样的环境中生存非常重要。然而，关于免疫系统发育的知识被认为是有限的。在许多物种中，溶菌酶被视为一线免疫防御的重要基因。先前报告中对溶菌酶mRNA的早期检测，引发了对其在卵母细胞中存在情况的研究。结果，在未受精的卵母细胞中检测到了c型溶菌酶mRNA转录本，表明存在母源传递。因此，我们研究了牙鲆中溶菌酶的表达模式，包括成熟卵母细胞中的表达模式。在我们的研究结果中，c型溶菌酶mRNA在未受精卵母细胞阶段首次被检测到，在孵化阶段之前显著下降，在孵化阶段之后显著增加。另一方面，g型溶菌酶mRNA转录本在神经胚晚期首次被检测到，在孵化后20天mRNA水平显著增加。这可能表明母源提供的mRNA在胚胎转录激活之前被选择性降解。这项研究将有助于理解牙鲆免疫系统发育过程中体液免疫的成熟和起始。
