Yang Hyun, Lee Young Mee, Lee Jeong-Ho, Noh Jae Koo, Kim Hyun Chul, Park Choul-Ji, Park Jong-Won, Hwang In Joon, Kim Sung Yeon
Genetics and Breeding Research Center, NFRDI, Geoje 656-842, Republic of Korea.
Dev Reprod. 2013 Dec;17(4):321-7. doi: 10.12717/DR.2013.17.4.321.
The innate immune system is the only defense weapon that invertebrates have, and it is the fundamental defense mechanism for fish. The innate immune response is important in newly hatched flounders because it is closely involved in the initial feeding phase, which is why it is essential for survival during the juvenile period. The expression analysis of genes involved in the innate immune response in the olive flounder (Paralichthys olivaceus) in the days after hatching is incomplete. Therefore, we have begun to examine the expression patterns of genes specifically induced during the development of the innate immune system in newly hatched flounders. Microscopic observation showed that pronephron formation corresponded with the expression of perforin-encoding gene. These results suggest that perforin plays a vital role in the innate immunity of the kidney during developmental stages. Perforin expression was strong at the start of the development of the innate immune response, and continued throughout all the development stages. Our findings have important implications with respect to perforin's biological role and the evolution of the first defense mechanisms in olive flounder. Further studies are required to elucidate the perforin-mediated innate immunity response and to decipher the functional role of perforin in developmental stages.
先天免疫系统是无脊椎动物仅有的防御武器，也是鱼类的基本防御机制。先天免疫反应在新孵化的比目鱼中很重要，因为它与初始摄食阶段密切相关，这就是为什么它对幼鱼期的生存至关重要。孵化后数天内，牙鲆（Paralichthys olivaceus）先天免疫反应相关基因的表达分析并不完整。因此，我们开始研究新孵化牙鲆先天免疫系统发育过程中特异性诱导基因的表达模式。显微镜观察表明，前肾形成与穿孔素编码基因的表达相对应。这些结果表明，穿孔素在发育阶段的肾脏先天免疫中起着至关重要的作用。穿孔素表达在先天免疫反应开始时很强，并在所有发育阶段持续存在。我们的发现对于穿孔素的生物学作用以及牙鲆第一道防御机制的进化具有重要意义。需要进一步研究以阐明穿孔素介导的先天免疫反应，并破译穿孔素在发育阶段的功能作用。
